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Titolo:
Improved detection of viral RNA isolated from liquid-based cytology samples
Autore:
Tarkowski, TA; Rajeevan, MS; Lee, DR; Unger, ER;
Indirizzi:
CDCP, Div Viral & Rickettsial Dis, Viral Exanthems & Herpesvirus Branch, Atlanta, GA 30333 USA CDCP Atlanta GA USA 30333 ems & Herpesvirus Branch, Atlanta, GA 30333 USA
Titolo Testata:
MOLECULAR DIAGNOSIS
fascicolo: 2, volume: 6, anno: 2001,
pagine: 125 - 130
SICI:
1084-8592(200106)6:2<125:IDOVRI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; TRANSCRIPTS;
Keywords:
molecular epidemiology; RNA extraction; HPV; E6/E7 RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Unger, ER CDCP, Div Viral & Rickettsial Dis, Viral Exanthems & HerpesvirusBranch, 1600 Clifton Rd,MSG18, Atlanta, GA 30333 USA CDCP 1600 Clifton Rd,MSG18 Atlanta GA USA 30333 ta, GA 30333 USA
Citazione:
T.A. Tarkowski et al., "Improved detection of viral RNA isolated from liquid-based cytology samples", MOL DIAGN, 6(2), 2001, pp. 125-130

Abstract

Background: Molecular diagnosis requires the ability to obtain high-quality nucleic acids that are representative of the disease state. We evaluated the recovery and detection of limiting amounts of viral oncogenic RNA from cells fixed in liquid-based cytology media. Methods and Results: Serial dilutions of a human papillomavirus (HPV)-positive cell line fixed in a liquid media was used as a model system. Total nucleic acid (TNA) extraction produced RNA with clearly visible ribosomal bands even after one year of storage. These TNA extracts, treated with DNase-I, were used in an RT-PCR assay for HPV-16 E6-E7 oncogenic transcripts. Withchemiluminscent Southern blot detection, samples with one HPV-positive cell in 30,000 were consistently detected. Conclusion: PreservCyt-fixed cells can yield RNA suitable for molecular assays even after one year of storage.

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Documento generato il 06/04/20 alle ore 20:02:43