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Titolo:
Transcriptional complementarity in breast cancer: Application to detectionof circulating tumor cells
Autore:
Houghton, RL; Dillion, DC; Molesh, DA; Zehentner, BK; Xu, JC; Jiang, J; Schmidt, C; Frudakis, A; Repasky, E; Maltez, A; Nolasco, M; Badaro, R; Zhang, XQ; Roche, PC; Persing, DH; Reed, SG;
Indirizzi:
Corixa Corp, Seattle, WA 98104 USA Corixa Corp Seattle WA USA 98104Corixa Corp, Seattle, WA 98104 USA DNAPrint Genom, Sarasota, FL USA DNAPrint Genom Sarasota FL USADNAPrint Genom, Sarasota, FL USA Roswell Pk Canc Inst, Buffalo, NY 14263 USA Roswell Pk Canc Inst Buffalo NY USA 14263 anc Inst, Buffalo, NY 14263 USA Mayo Clin, Dept Pathol & Lab Med, Rochester, MN USA Mayo Clin Rochester MN USA lin, Dept Pathol & Lab Med, Rochester, MN USA Hosp Aristides Maltez, Salvador, BA, Brazil Hosp Aristides Maltez Salvador BA Brazil es Maltez, Salvador, BA, Brazil
Titolo Testata:
MOLECULAR DIAGNOSIS
fascicolo: 2, volume: 6, anno: 2001,
pagine: 79 - 91
SICI:
1084-8592(200106)6:2<79:TCIBCA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HER-2/NEU ONCOGENIC PROTEIN; UTEROGLOBIN GENE FAMILY; PERIPHERAL-BLOOD; MAMMARY-CARCINOMA; SENSITIVE METHOD; RT-PCR; MAMMAGLOBIN; POLYMERASE; SYSTEM; RECEPTOR;
Keywords:
breast cancer; genes; quantitative PCR; complementation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Houghton, RL Corixa Corp, 1124 Columbia St,Ste 200, Seattle, WA 98104 USA Corixa Corp 1124 Columbia St,Ste 200 Seattle WA USA 98104 USA
Citazione:
R.L. Houghton et al., "Transcriptional complementarity in breast cancer: Application to detectionof circulating tumor cells", MOL DIAGN, 6(2), 2001, pp. 79-91

Abstract

Background: We used a combination of genetic subtraction, silicon DNA microarray analysis, and quantitative PCR to identify tissue- and tumor-specific genes as diagnostic targets for breast cancer. Methods and Results: From a large number of candidate antigens, several specific subsets of genes were identified that showed concordant and complementary expression profiles. Whereas transcriptional profiling of mammaglobinresulted in the detection of 70% of tumors in a panel of 46 primary and metastatic breast cancers, the inclusion of three additional markers resultedin detection of all 46 specimens. Immunomagnetic epithelial cell enrichment of circulating tumor cells from the peripheral blood of patients with metastatic breast cancer, coupled with RT PCR-based amplification of breast tumor-specific transcripts, resulted in the detection of anchorage-independent tumor cells in the majority of patients with breast cancer with known metastatic disease. Conclusion: Complementation of mammaglobin with three additional genes in RT-PCR increases the detection of breast cancers in tissue and circulating tumor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:32:07