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Titolo:
Characterization of changes in IgG associated oligosaccharide profiles in rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis usingfluorophore linked carbohydrate electrophoresis
Autore:
Martin, K; Talukder, R; Hay, FC; Axford, JS;
Indirizzi:
St George Hosp, Sch Med, Acad Unit Musculoskeletal Dis, Dept Biochem & Immunol, London SW17 0RE, England St George Hosp London England SW17 0RE Immunol, London SW17 0RE, England
Titolo Testata:
JOURNAL OF RHEUMATOLOGY
fascicolo: 7, volume: 28, anno: 2001,
pagine: 1531 - 1536
SICI:
0315-162X(200107)28:7<1531:COCIIA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOSYLATION CHANGES; AGALACTOSYL IGG; SERUM IGG; GALACTOSE; BINDING; REDUCTION; JUVENILE; DISEASES; PROTEIN; LECTIN;
Keywords:
rheumatoid arthritis; psoriatic arthritis; ankylosing spondylitis; immunoglobulin G; oligosaccharide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Axford, JS St George Hosp, Sch Med, Acad Unit Musculoskeletal Dis, Dept Biochem & Immunol, Cranmer Terrace, London SW17 0RE, England St George Hosp Cranmer Terrace London England SW17 0RE England
Citazione:
K. Martin et al., "Characterization of changes in IgG associated oligosaccharide profiles in rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis usingfluorophore linked carbohydrate electrophoresis", J RHEUMATOL, 28(7), 2001, pp. 1531-1536

Abstract

Objective. To investigate fluorophore linked carbohydrate electrophoresis (FCE) as a method of analyzing serum immunoglobulin G (IgG) oligosaccharides in healthy individuals and those with rheumatic disease and compare with lectin binding assays of carbohydrate composition. Methods, IgG was isolated from patients with rheumatoid arthritis (RA) (n = 21), ankylosing spondylitis (AS) (n = 20), psoriatic arthritis (PsA) (n =20), and healthy adults (n = 36). IgG oligosaccharides were released enzymatically, fluorescently labelled using 8 aminonaphthalene-136 trisulfonic acid; and identification of the oligosaccharide bands was by stepwise enzymatic degradation. Comparison of FCE was made with lectin binding analysis inwhich the lectins Ricinus communis (RCA1) and Bandeiraea simplicifolia (BSII) were used to detect galactose (Gal) and N-acetylglucosamine (GlcNAc), respectively. Results, Each disease could be differentiated from healthy adults on the basis of Band 1 asialodigalacto core fucosylated oligosaccharide (gf2) intensity (p = 0.001), but not from each other. Reduced levels of different sugars were associated with specific diseases: reduced gf2 with RA (p < 0.001),PsA (p < 0.001) and AS (p < 0.02), reduced Band 5 disialo-digalacto core fucosylated (a2f) oligosaccharide with AS (p < 0.001), reduced Band 6 disialo-digalacto (a2) oligosaccharide with AS (p < 0.001) and PsA (p = 0.021). AH diseases were associated with a significant increase in Band 4 asialo-agalacto core fucosylated oligosaccharide (g0f) (p < 0.001). In RA, FCE band intensities correlated with sugar quantity when identified using lectin binding analysis (p < 0.003). In contrast, there was no correlation between thesame bands in healthy individuals. Conclusion. FCE is an accurate method of analyzing IgG associated oligosaccharides and reveals unique band patterns or sugar prints associated with healthy adults and patients with RA, PsA,and AS, and comparison with lectin binding analysis suggests undetected RAglycoprotein structural differences. FCE has potential in the early diagnosis and differentiation of rheumatic diseases.

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Documento generato il 29/09/20 alle ore 00:08:01