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Titolo:
Determination of retinoids in human serum, tocopherol and retinyl acetate in pharmaceuticals by RP-LC with electrochemical detection
Autore:
Wang, LH; Wang, JF;
Indirizzi:
China Nan Univ Pharm & Sci, Dept Appl Chem, Tainan 71710, Taiwan China NanUniv Pharm & Sci Tainan Taiwan 71710 hem, Tainan 71710, Taiwan
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 5-6, volume: 25, anno: 2001,
pagine: 785 - 793
SICI:
0731-7085(200107)25:5-6<785:DORIHS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ALL-TRANS-RETINOL; 13-CIS-RETINOIC ACID; ULTRAVIOLET DETECTION; 4-OXO METABOLITES; BETA-CAROTENE; HUMAN-PLASMA; VITAMIN-A; FLUID; EXTRACTION;
Keywords:
retinoids; tocopherol acetate; human serum; pharmaceuticals; electrochemical detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, LH China Nan Univ Pharm & Sci, Dept Appl Chem, Tainan 71710, Taiwan China Nan Univ Pharm & Sci Tainan Taiwan 71710 an 71710, Taiwan
Citazione:
L.H. Wang e J.F. Wang, "Determination of retinoids in human serum, tocopherol and retinyl acetate in pharmaceuticals by RP-LC with electrochemical detection", J PHARM B, 25(5-6), 2001, pp. 785-793

Abstract

A liquid chromatography with a electrochemical detector method has been developed of the quantitative measurement for the three retinoids in human serum (13-cis and all-trans retinoic acid and retinol), as well as tocopherolacetate retinyl acetate and retinol in pharmaceuticals. The detection cellconsisted of a glassy carbon electrode held at 1.0 V versus an Ag/AgCl reference electrode. The maximum electrochemical signal was obtained with a supporting electrolyte containing 92%, methanol 0.1 M acetate buffer (pH 4.72) as the mobile phase. The quantification limits are 0.5. 0.2, 0.5, 0.5, 0.8 and 0.8 ng for tocopherol acetate, all-trans-RA, 13-cis-RA, retinol, retinal and retinyl acetate, respectively. The electrooxidation process is applied for the simultaneous quantitative determination of retinoids in human serum. Comparison with results obtained From HPLC-UV shows agreement. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:38:28