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Titolo:
Spontaneous changes in mitochondrial membrane potential in cultured neurons
Autore:
Buckman, JF; Reynolds, IJ;
Indirizzi:
Univ Pittsburgh, Dept Pharmacol, Pittsburgh, PA 15261 USA Univ PittsburghPittsburgh PA USA 15261 armacol, Pittsburgh, PA 15261 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 14, volume: 21, anno: 2001,
pagine: 5054 - 5065
SICI:
0270-6474(20010715)21:14<5054:SCIMMP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT FOREBRAIN NEURONS; CEREBELLAR GRANULE CELLS; GLUTAMATE EXCITOTOXICITY; PERMEABILITY TRANSITION; HIPPOCAMPAL-NEURONS; OXIDATIVE STRESS; CORTICAL-NEURONS; CYCLOSPORINE-A; CALCIUM; APOPTOSIS;
Keywords:
mitochondria; membrane potential; ATP; JC-1; TMRM; F1F0 ATPase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Reynolds, IJ Univ Pittsburgh, Dept Pharmacol, W1351 Biomed Sci Tower, Pittsburgh, PA 15261 USA Univ Pittsburgh W1351 Biomed Sci Tower Pittsburgh PA USA 15261
Citazione:
J.F. Buckman e I.J. Reynolds, "Spontaneous changes in mitochondrial membrane potential in cultured neurons", J NEUROSC, 21(14), 2001, pp. 5054-5065

Abstract

Using the mitochondrial membrane potential (Delta psi (m))-sensitive fluorescent dyes 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide (JC-1) and tetramethylrhodamine methyl ester (TMRM), we have observed spontaneous changes in the Delta psi (m) of cultured forebrain neurons. These fluctuations in Delta psi (m) appear to represent partial, transient depolarizations of individual mitochondria. The frequency of these Delta psi (m), fluctuations can be significantly lowered by exposure to a photo-induced oxidant burden, an ATP synthase inhibitor, or a glutamate-induced sodium load, without changing overall JC-1 fluorescence intensity. These spontaneous fluctuations in JC-1 signal were not inhibited by altering plasmamembrane activity with tetrodotoxin or MK-801 or by blocking the mitochondrial permeability transition pore (PTP) with cyclosporin A. Neurons loaded with TMRM showed similar, low-amplitude, spontaneous fluctuations in Delta psi (m). We hypothesize that these Delta psi (m) fluctuations are dependenton the proper functioning of the mitochondria and reflect mitochondria alternating between the active and inactive states of oxidative phosphorylation.

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Documento generato il 04/04/20 alle ore 12:09:10