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Titolo:
2-Chloro-1-methylquinolinium tetrafluoroborate as an effective and thiol specific uv-tagging reagent for liquid chromatography
Autore:
Bald, E; Glowacki, R;
Indirizzi:
Univ Lodz, Dept Environm Chem, PL-90236 Lodz, Poland Univ Lodz Lodz Poland PL-90236 Dept Environm Chem, PL-90236 Lodz, Poland
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 9, volume: 24, anno: 2001,
pagine: 1323 - 1339
SICI:
1082-6076(2001)24:9<1323:2TAAEA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTCOLUMN DERIVATIZATION; FLUOROGENIC REAGENT; HUMAN PLASMA; HOMOCYSTEINE; CYSTEINE; ASSAY; CAPTOPRIL; AMMONIUM; URINE; HPLC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Bald, E Univ Lodz, Dept Environm Chem, 163 Pomorska Str, PL-90236 Lodz, Poland Univ Lodz 163 Pomorska Str Lodz Poland PL-90236 0236 Lodz, Poland
Citazione:
E. Bald e R. Glowacki, "2-Chloro-1-methylquinolinium tetrafluoroborate as an effective and thiol specific uv-tagging reagent for liquid chromatography", J LIQ CHR R, 24(9), 2001, pp. 1323-1339

Abstract

A new highly reactive, and thiol specific derivatization reagent for liquid chromatographic analysis has been developed. The reagent, 2-chloro-1-methylquinolinium tetrafluoroburate, reacts instantaneously with hydrophilic thiols in water under mild conditions. The 2-S-quinolinium derivatives, resulting from this reaction, are stable thioethers exhibiting well defined absorption maximum at 348 nm and molar absorptivity coefficient about 2 x 10(4)L mol(-1) cm(-1). It is shown that under reversed-phase high performance liquid chromatography conditions with gradient elution, six thiols possessing different functional groups, can be baseline separated and quantified within 4 min in one analytical run. The lower limits of detection and quantitation of the analytes (20 muL injection volume) are within 0.3-1.5 pmol and 0.5-4.0 pmol, respectively. The assays are linear in the range of 0.025-8 nmol/mL with correlation coefficients values close to 0.9999. Synthesis of 2-chloro-1-methylquinolinium tetrafluoroborate, as well as isolation of the 2-S-quinolinium derivative of one model thiol, are described.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:30:57