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Titolo:
A simple method for culturing mouse vascular endothelium
Autore:
Kreisel, D; Krupnick, AS; Szeto, WY; Popma, SH; Sankaran, D; Krasinskas, AM; Amin, KM; Rosengard, BR;
Indirizzi:
Univ Penn, Med Ctr, Div Cardiothorac Surg, Dept Surg, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Dept Surg, Philadelphia, PA 19104 USA Univ Penn, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PAUSA 19104 Dept Med, Philadelphia, PA 19104 USA Univ Penn, Med Ctr, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA UnivPenn Philadelphia PA USA 19104 & Lab Med, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 254, anno: 2001,
pagine: 31 - 45
SICI:
0022-1759(20010801)254:1-2<31:ASMFCM>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION MOLECULE-1; TUMOR-NECROSIS-FACTOR; T-CELLS; TRANSENDOTHELIAL MIGRATION; EXPRESSION; COMPLEX; LEUKOCYTES; ACTIVATION; ANTIGENS; ENDOGLIN;
Keywords:
endothelial cells; mouse; culture methods;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Rosengard, BR Univ Penn, Med Ctr, Div Cardiothorac Surg, Dept Surg, 6 Silverstein,3400 Spruce, Philadelphia, PA 19104 USA Univ Penn 6 Silverstein,3400 Spruce Philadelphia PA USA 19104
Citazione:
D. Kreisel et al., "A simple method for culturing mouse vascular endothelium", J IMMUNOL M, 254(1-2), 2001, pp. 31-45

Abstract

Vascular endothelium is an important site for a wide array of immunological processes such as inflammation, atherosclerosis and allograft rejection. Culture methods of mouse vascular endothelium would provide an important invitro correlate to immunological murine in vivo models. We describe a simple method to culture mouse vascular endothelium from thoracic aorta. Our cultured cells express typical phenotypic (CD105, CD31, CD106), morphologicaland ultrastructural (intercellular junctions, Weibel-Palade bodies) markers of vascular endothelium. They also possess functional receptors for uptake and processing of acetylated low-density lipoproteins. The mouse vascularendothelium within our system expresses high levels of MHC class I and MHCclass II after activation with IFN-gamma, In addition, these cells expressthe accessory molecules CD80 and CD54, while they lack constitutive expression of CD86 and CD40, providing them the means to function as antigen presenting cells. Alloreactive CD4(+) and CD8(+) T lymphocytes demonstrate evidence of DNA synthesis after co-culture with activated vascular endothelium indicating their commitment to proliferation. In conclusion, we describe a simple culture system to isolate and grow mouse vascular endothelium, whichprovides a powerful tool to study biological interactions in vitro. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 17:42:29