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Titolo:
Quantitative assessment of retinoid signaling pathways in the developing eye and retina of the chicken embryo
Autore:
Hoover, F; Gundersen, TE; Ulven, SM; Michaille, JJ; Blanchet, S; Blomhoff, R; Glover, JC;
Indirizzi:
Univ Oslo, Inst Basic Med Sci, Dept Anat, N-0317 Oslo, Norway Univ Oslo Oslo Norway N-0317 sic Med Sci, Dept Anat, N-0317 Oslo, Norway Univ Oslo, Inst Basic Med Sci, Inst Nutr Res, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316 Med Sci, Inst Nutr Res, N-0316 Oslo, Norway Univ Grenoble 1, Lab Biol Differenciat Epitheliale, Grenoble, France Univ Grenoble 1 Grenoble France ferenciat Epitheliale, Grenoble, France
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 3, volume: 436, anno: 2001,
pagine: 324 - 335
SICI:
0021-9967(20010730)436:3<324:QAORSP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; PERFORMANCE LIQUID-CHROMATOGRAPHY; RECEPTOR-GAMMA TRANSCRIPTS; ACID RECEPTOR; DIFFERENTIAL EXPRESSION; ENDOGENOUS RETINOIDS; SKIN DEVELOPMENT; NERVOUS-SYSTEM; MUTANT MICE; ALPHA GENE;
Keywords:
vitamin A; retinoic acid; retinol; RALDH2; retinoic acid receptor; RXR; RAR; retina; neuronal differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Glover, JC Univ Oslo, Inst Basic Med Sci, Dept Anat, POB 1105, N-0317 Oslo, Norway Univ Oslo POB 1105 Oslo Norway N-0317 105, N-0317 Oslo, Norway
Citazione:
F. Hoover et al., "Quantitative assessment of retinoid signaling pathways in the developing eye and retina of the chicken embryo", J COMP NEUR, 436(3), 2001, pp. 324-335

Abstract

Retinoid signaling has been implicated as an important regulator of retinal development and differentiation. We have used state of the art high-pressure liquid chromatography to identify and quantitate biologically active retinoids, immunohistochemistry to localize the retinoic acid synthetic enzyme retinaldehyde dehydrogenase 2 (RALDH2), and nucleic acid assays to quantitate and localize retinoid receptor gene transcripts in the developing eye and retina of the chicken. Our results demonstrate spatial distinctions in retinoid synthesis and signaling that may be related to laminar differentiation in the developing retina. Retinoic acids (RAs) and their precursor retinols (ROHs) are the predominant retinoids in the developing eye. All-trans-RA and all-trans-3,4-didehydro-RA are present in the neuroepithelium in approximately equal amounts from early stages of neurogenesis until shortly before hatching. The retinoid X receptor (RXR) ligand 9-cis-RA is undetectable at all stages; if present, it cannot exceed a small percentage of the total RA content. RAs are not detected in the pigment epithelium. All-trans-ROH is present in the neuroepithelium and pigment epithelium, whereas all-trans-3,4-didehydro-ROH is detected only in the pigment epithelium and/or thechoroid and sclera. RALDH2 immunoreactivity is intense in the choroid, lowor absent in the pigment epithelium, and moderate in the neuroepithelium, where it is highest in the outer layers. Transcripts of all five chicken retinoid receptor genes are present in the neural retina and eye throughout development. During the period of neurogenesis, at least three of the receptors (RAR gamma, RXR gamma, RXR alpha), exhibit dynamic patterns of differential localization within the depths of the neural retina. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 04:05:13