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Titolo:
Direct analysis of urinary trans,trans-muconic acid by coupled column liquid chromatography and spectrophotometric ultraviolet detection: method applicability to human urine
Autore:
Marrubini, G; Coccini, T; Manzo, L;
Indirizzi:
Univ Pavia, Dept Internal Med, Clin Toxicol Div, Lab Pharmacokinet & Analyt Toxicol, I-27028 San Martino Siccomario, Pavia, Italy Univ Pavia San Martino Siccomario Pavia Italy I-27028 mario, Pavia, Italy Inst Pavia, Salvatore Maugeri Fdn, Div Toxicol, Pavia, Italy Inst Pavia Pavia Italy Salvatore Maugeri Fdn, Div Toxicol, Pavia, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 758, anno: 2001,
pagine: 295 - 303
SICI:
1387-2273(20010715)758:2<295:DAOUTA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
S-PHENYLMERCAPTURIC ACID; BENZENE EXPOSURE; MUCONIC ACID; BIOLOGICAL INDICATOR; MASS-SPECTROMETRY; T,T-MUCONIC ACID; BIOMARKERS; PHENOL; LEVEL; METABOLITES;
Keywords:
muconic acid; benzene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Marrubini, G Univ Pavia, Dept Internal Med, Clin Toxicol Div, Lab Pharmacokinet & Analyt Toxicol, Via Brodolini 7, I-27028 San Martino Siccomario, Pavia, Italy Univ Pavia Via Brodolini 7 San Martino Siccomario Pavia Italy I-27028
Citazione:
G. Marrubini et al., "Direct analysis of urinary trans,trans-muconic acid by coupled column liquid chromatography and spectrophotometric ultraviolet detection: method applicability to human urine", J CHROMAT B, 758(2), 2001, pp. 295-303

Abstract

A coupled column liquid chromatographic (LC-LC) method for the direct analysis in human urine of the ring opened benzene metabolite, trans.trans-muconic acid (t,t-MA) is described. The method was tested using urine samples collected from five refinery workers exposed to low concentrations of airborne benzene (0.2-0.5 ppm), and from non-exposed volunteers. The analytical columns used were of 50X4.6 mm I.D. packed with 3 mum p.s. Microspher C-18 material as the first column (C-1), and a 100X4.6 mm I.D. column packed with3 mum p.s. Hypersil ODS material as the second one (C-2). The mobile phases applied consisted, respectively, of methanol-0.074% trifluoroacetic acid (TFA) in water (4:96, v/v) on C-1, and of methanol-0.074% TFA in water (10:90, v/v) on C-2. Under these conditions t.t-MA eluted 15 min after injection. The present method, coupling the LC-LC technique with UV detection at 264 nm, permits the quantitation of t,t-MA directly in urine at levels as lowas 0.05 mg/l. The determination is performed with a sample throughput of 2h(-1) requiring only pH adjustment and centrifugation of the sample. Calibration plots of standard additions of t,t-MA to pooled urine taken from five non-exposed subjects were linear (r >0.9991 over a wide concentration range (0.05, 0.1, 0.5, 1.0, 2.0 mg/l). The precision of the method (RSD) was in the range of 0.5 to 3.8%, and the within-session repeatability on workersurine samples (levels 0.06, 0.1, 0.2. 1.0 mg/l) was in the range of 3 to 8%. The present method improves the applicability of routine t,t-MA analysis, where it is most desirable that a large number of biological samples can be processed automatically or with minimal human labour, at low cost, and with a convenient turn-around time. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:17:39