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Titolo:
Pre-column derivatization high-performance liquid chromatographic method for determination of cysteine, cysteinyl-glycine, homocysteine and glutathione in plasma and cell extracts
Autore:
Katrusiak, AE; Paterson, PG; Kamencic, H; Shoker, A; Lyon, AW;
Indirizzi:
Univ Saskatchewan, Coll Med, Dept Pathol, Saskatoon, SK S7N 0W0, Canada Univ Saskatchewan Saskatoon SK Canada S7N 0W0 skatoon, SK S7N 0W0, Canada Coll Pharm & Nutr, Saskatoon, SK, Canada Coll Pharm & Nutr Saskatoon SK Canada harm & Nutr, Saskatoon, SK, Canada Univ Saskatchewan, Coll Med, Dept Anat & Cell Biol, Saskatoon, SK S7N 0W0,Canada Univ Saskatchewan Saskatoon SK Canada S7N 0W0 askatoon, SK S7N 0W0,Canada Univ Saskatchewan, Coll Med, Dept Med, Saskatoon, SK S7N 0W0, Canada Univ Saskatchewan Saskatoon SK Canada S7N 0W0 skatoon, SK S7N 0W0, Canada Dept Med, Saskatoon, SK, Canada Dept Med Saskatoon SK CanadaDept Med, Saskatoon, SK, Canada Dept Lab Med, Saskatoon, SK, Canada Dept Lab Med Saskatoon SK CanadaDept Lab Med, Saskatoon, SK, Canada
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 758, anno: 2001,
pagine: 207 - 212
SICI:
1387-2273(20010715)758:2<207:PDHLCM>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
REDOX STATUS; ELECTROCHEMICAL DETECTION; FLUORESCENCE DETECTION; HEALTHY-SUBJECTS; THIOLS; LIVER; HPLC;
Keywords:
cysteine; cysteinyl-glycine; homocysteine; glutathione;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Lyon, AW Calgary Lab Serv, 1638 10th Ave SW, Calgary, AB T3C OJ5, Canada Calgary Lab Serv 1638 10th Ave SW Calgary AB Canada T3C OJ5 anada
Citazione:
A.E. Katrusiak et al., "Pre-column derivatization high-performance liquid chromatographic method for determination of cysteine, cysteinyl-glycine, homocysteine and glutathione in plasma and cell extracts", J CHROMAT B, 758(2), 2001, pp. 207-212

Abstract

A sensitive high-performance liquid chromatographic method for quantification of sulphydryl and disulfide amino acids in human plasma using ultra violet spectrophotometric detection was developed. Precolumn derivatization with 5,5 ' -dithio-bisnitrobenzoic acid (DTNB) and an optional pre-derivatization reaction with dithiothreitol allowed both quantitative reduction of disulfides for measurement of total amino acid levels and the measurement of the reduced forms, A dynamic range of 500 nmol/1-750 mu mol/l allowed the major analytes of interest to be quantified in plasma without sample dilution. The assay is a sensitive and precise method for the determination of sulphydryl and disulfide amino acids in plasma and cell extracts. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:26:26