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Titolo:
Determination of 2-hydroxyflutamide in human plasma by high-performance liquid chromatography and its application to pharmacokinetic studies
Autore:
Niopas, I; Daftsios, AC;
Indirizzi:
Aristotelian Univ Salonika, Sch Hlth Sci, Dept Pharm, GR-54006 Salonika, Greece Aristotelian Univ Salonika Salonika Greece GR-54006 006 Salonika, Greece Amer Farm Sch Thessaloniki, Thessaloniki 55102, Greece Amer Farm Sch Thessaloniki Thessaloniki Greece 55102 oniki 55102, Greece
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 759, anno: 2001,
pagine: 179 - 183
SICI:
1387-2273(20010805)759:1<179:DO2IHP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTATE-CANCER; FLUTAMIDE; HYDROXYFLUTAMIDE; BIOEQUIVALENCE;
Keywords:
2-hydroxyflutamide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Niopas, I Aristotelian Univ Salonika, Sch Hlth Sci, Dept Pharm, GR-54006 Salonika, Greece Aristotelian Univ Salonika Salonika Greece GR-54006 ka, Greece
Citazione:
I. Niopas e A.C. Daftsios, "Determination of 2-hydroxyflutamide in human plasma by high-performance liquid chromatography and its application to pharmacokinetic studies", J CHROMAT B, 759(1), 2001, pp. 179-183

Abstract

dFlutamide is a potent antiandrogen used for the treatment of prostatic cancer. Flutamide undergoes extensive first-pass metabolism to the pharmacologically active metabolite 2-hydroxyflutamide. A simple, sensitive, precise,accurate and specific HPLC method, using carbamazepine as the internal standard, for the determination of 2-hydroxyflutamide in human plasma was developed and validated. After addition of the internal standard, the analytes were isolated from human plasma by liquid-liquid extraction. The method waslinear in the 25 to 1000 ng/ml concentration range (r>0.999). Recovery for2-hydroxyflutamide was greater than 91.4% and for internal standard was 93.6%. The limit of quantitation was 25 ng/ml. Inter-batch precision, expressed as the relative standard deviation (RSD), ranged from 4.3 to 7.9%, and accuracy was better than 93.9%. Analysis of 2-hydroxyflutamide concentrations in plasma samples from 16 healthy volunteers following oral administration of 250 mg of flutamide provided the following pharmacokinetic data (mean /- SD): C-max, 776 +/- 400 ng/ml; AUC(0-infinity), 5368 +/- 2689 ng h/ml; AUC(0-t), 5005 +/- 2605 ng h/ml; T-max, 2.6 +/-1.6 h; elimination half-life, 5.2 +/-2.0 h. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 15:57:57