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Titolo:
Determination of rutin in human plasma by high-performance liquid chromatography utilizing solid-phase extraction and ultraviolet detection
Autore:
Ishii, K; Furuta, T; Kasuya, Y;
Indirizzi:
Kyorin Univ, Sch Hlth Sci, Hachioji, Tokyo 1920005, Japan Kyorin Univ Hachioji Tokyo Japan 1920005 , Hachioji, Tokyo 1920005, Japan Tokyo Univ Pharm & Life Sci, Hachioji, Tokyo 1920392, Japan Tokyo Univ Pharm & Life Sci Hachioji Tokyo Japan 1920392 o 1920392, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 759, anno: 2001,
pagine: 161 - 168
SICI:
1387-2273(20010805)759:1<161:DORIHP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER; FLAVONOIDS; QUERCETIN; ANTIOXIDANT; IDENTIFICATION; BIOAVAILABILITY; GLYCOSIDES; INHIBITION; GENISTEIN; NARINGIN;
Keywords:
rutin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ishii, K Kyorin Univ, Sch Hlth Sci, 476 Miyasita, Hachioji, Tokyo 1920005,Japan Kyorin Univ 476 Miyasita Hachioji Tokyo Japan 1920005 0005, Japan
Citazione:
K. Ishii et al., "Determination of rutin in human plasma by high-performance liquid chromatography utilizing solid-phase extraction and ultraviolet detection", J CHROMAT B, 759(1), 2001, pp. 161-168

Abstract

An HPLC method for determining a flavonol glycoside, rutin, in human plasma is presented for application to the pharmacokinetic study. Isocratic reversed-phase HPLC was employed for the quantitative analysis by using kaempferol-3-rutinoside as an internal standard. Solid-phase extraction was performed on an Oasis MAX cartridge possessing reversed-phase and anion-exchange functions (recovery, approximately 80%). The HPLC assay was carried out using a Luna ODS-2 column (150X2.1 mm I.D., 5 mum particle size). The mobile phase was acetonitrile-10 mM ammonium acetate solution containing 0.3 mM EDTA-glacial acetic acid (16.5:82.5:1, v/v, pH 3.8). The flow-rate was 0.3 ml/min. The detection wavelength was set at 370 nm. Calibration of the overallanalytical procedure gave a Linear signal (r>0.9999) over a concentration range of 3-1000 ng/ml of rutin in plasma. The lower limit of quantificationwas ca. 5 ng/ml of rutin in plasma. The detection Limit (defined as signal-to-noise ratio of about 3) was approximately 0.75 ng/ml. A preliminary experiment to investigate the plasma concentration of rutin after oral administration of 500 mg of rutin to a healthy volunteer demonstrated that the present method was suitable for determining rutin in human plasma. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 22:36:28