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Titolo:
Signal transduction in eclosion hormone-induced secretion of ecdysis-triggering hormone
Autore:
Kingan, TG; Cardullo, RA; Adams, ME;
Indirizzi:
Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 omol, Riverside, CA 92521 USA Univ Calif Riverside, Dept Biol, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 Biol, Riverside, CA 92521 USA Univ Calif Riverside, Dept Cell Biol & Neurosci, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 osci, Riverside, CA 92521 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 27, volume: 276, anno: 2001,
pagine: 25136 - 25142
SICI:
0021-9258(20010706)276:27<25136:STIEHS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT PROTEIN-KINASE; MANDUCA-SEXTA; NERVOUS-SYSTEM; NITRIC-OXIDE; CALYCULIN-A; BEHAVIORAL SEQUENCE; ENDOCRINE SYSTEM; CHROMAFFIN CELLS; INSECT ECDYSIS; CA2+ STORES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Kingan, TG Univ Calif Riverside, Dept Entomol, 5419 Boyce Hall, Riverside,CA 92521 USA Univ Calif Riverside 5419 Boyce Hall Riverside CA USA 92521 USA
Citazione:
T.G. Kingan et al., "Signal transduction in eclosion hormone-induced secretion of ecdysis-triggering hormone", J BIOL CHEM, 276(27), 2001, pp. 25136-25142

Abstract

Inks cells of insect epitracheal glands (EGs) secrete preecdysis and ecdysis-triggering hormones (PETH and ETH) at the end of each developmental stage. Both peptides act in the central nervous system to evoke the ecdysis behavioral sequence, a stereotype behavior during which old cuticle is shed. Secretion of ETH is stimulated by a brain neuropeptide, eclosion hormone (EH), EH evokes accumulation of cGMP followed by release of ETH from Inks cells, and exogenous cGMP evokes secretion of ETH, The secretory responses to EH and cGMP are inhibited by the broad-spectrum kinase inhibitor staurosporine, and the response to EH is potentiated by the phosphatase inhibitor calyculin A Staurosporine did not inhibit EH-evoked accumulation of cGMP, Changes in cytoplasmic Ca2+ in Inks cells during EH signaling were monitored viafluorescence ratioing with fura-2-loaded EGs. Cytoplasmic Ca2+ increases within 30-120 s after addition of EH to EGs, and it remains elevated for at least 10 min, corresponding with the time course of secretion. Secretion isincreased in dose-dependent manner by the Ca2+-ATPase inhibitor thapsigargin, a treatment that does not elevate glandular cGMP above basal levels. The secretory response to EH is partially inhibited in glands loaded with EGTA, while cGMP levels are unaffected. These findings suggest that EH activates second messenger cascades leading to cGMP accumulation and Ca2+ mobilization and/or influx and that both pathways are required for a full secretoryresponse, cGMP activates a staurosporine-inhibitable protein kinase, We propose that Ca2+ acts vis a parallel cascade with a time course that is similar to that for cGMP activation of a cGMP-dependent protein kinase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:25:37