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Titolo:
Predominant identification of RNA-binding proteins in Fas-induced apoptosis by proteome analysis
Autore:
Thiede, B; Dimmler, C; Siejak, F; Rudel, T;
Indirizzi:
Max Planck Inst Infektionsbiol, Abt Mol Biol, D-10117 Berlin, Germany Max Planck Inst Infektionsbiol Berlin Germany D-10117 17 Berlin, Germany
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 28, volume: 276, anno: 2001,
pagine: 26044 - 26050
SICI:
0021-9258(20010713)276:28<26044:PIORPI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS; LYMPHOMA CELL-LINE; HNRNP A/B PROTEINS; IN-VIVO; MEDIATED APOPTOSIS; MASS-SPECTROMETRY; IMMUNE PRIVILEGE; SPLICING FACTORS; DEATH; CASPASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Rudel, T Max Planck Inst Infektionsbiol, Abt Mol Biol, Schumannstr 21-22, D-10117 Berlin, Germany Max Planck Inst Infektionsbiol Schumannstr 21-22 Berlin Germany D-10117
Citazione:
B. Thiede et al., "Predominant identification of RNA-binding proteins in Fas-induced apoptosis by proteome analysis", J BIOL CHEM, 276(28), 2001, pp. 26044-26050

Abstract

Proteome analysis of Jurkat T cells was performed in order to identify proteins that are modified during apoptosis, Subtractive analysis of two-dimensional gel patterns of apoptotic and nonapoptotic cells revealed differences in 45 protein spots. 37 protein spots of 21 different proteins were identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization mass spectrometry, The hnRNPs A0, A2/B1, A3, K and R; the splicing factors p54(nrb), SRp30c, ASF-2, and KH-type splicing regulatory protein (FUSE-binding protein 2); and alpha NAG, NS1-associated protein 1, and poly(A)-binding protein 4 were hitherto unknown to be involved in apoptosis, The putative cleavage sites of the majority of the proteins could be calculated by the molecular masses and isoelectric points in the two-dimensional electrophoresis gel, the peptide mass fingerprints, and after translation by treatment with recombinant caspase-3, Remarkably, 15 of the 21 identifiedproteins contained the RNP or KH motif, the best characterized RNA-bindingmotifs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:13:55