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Titolo:
Apoptotic effects in primary human umbilical vein endothelial cell cultures caused by exposure to virion-associated and cell membrane-associated HIV-1 gp120
Autore:
Huang, MB; Khan, MF; Garcia-Barrio, M; Powell, M; Bond, VC;
Indirizzi:
Morehouse Sch Med, Dept Microbiol Biochem & Immunol, Atlanta, GA 30310 USAMorehouse Sch Med Atlanta GA USA 30310 m & Immunol, Atlanta, GA 30310 USA
Titolo Testata:
JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
fascicolo: 3, volume: 27, anno: 2001,
pagine: 213 - 221
SICI:
1525-4135(20010701)27:3<213:AEIPHU>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; CD4(+) T-CELLS; CHEMOKINE RECEPTOR CXCR4; ENVELOPE PROTEIN; NEURONAL APOPTOSIS; INFECTED PERSONS; LYMPH-NODES; INDEPENDENT MECHANISM; DISEASE PROGRESSION; AIDS PATHOGENESIS;
Keywords:
apoptosis; cell membrane; chemokine receptors; endothelium; gp 120; transfection; virus particles;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
77
Recensione:
Indirizzi per estratti:
Indirizzo: Bond, VC Morehouse Sch Med, Dept Microbiol Biochem & Immunol, 720 WestviewDr SW, Atlanta, GA 30310 USA Morehouse Sch Med 720 Westview Dr SW Atlanta GA USA 30310 310 USA
Citazione:
M.B. Huang et al., "Apoptotic effects in primary human umbilical vein endothelial cell cultures caused by exposure to virion-associated and cell membrane-associated HIV-1 gp120", J ACQ IMM D, 27(3), 2001, pp. 213-221

Abstract

During the course of HIV-I infection, free virus, infected cells, and freeHIV-I proteins circulate within the host, exposing the host endothelium tothese viral factors. We have previously presented evidence showing that soluble HIV-I gp120 protein interacts with chemokine receptors on primary human endothelium and (through those interactions) induces apoptosis as well as other intracellular effects. The current study examines the effect of exposure of vascular endothelium to gp120 IIIb expressed on the surface of Jurkat cells and in the context of viral particles. Apoptosis was observed in human umbilical vein endothelial cell (HUVEC) cultures exposed to gp 160-transfected Jurkat cells as well as to virion particles with gp 120 on their surface. Additional experiments show that this apoptotic effect was caused by gp120 protein acting through chemokine receptors on the HUVEC surface, primarily the CXCR4 receptor. At higher concentrations of gp120, this lymphotrophic variant, which has been shown to interact predominantly with CXCR4,seems to interact with and induce apoptosis through the CCR5 receptor. Finally, this apoptotic effect in HUVEC cultures occurs at low levels of the inducing agent, gp120, on cell membranes or on virion particles. These results demonstrate that HIV-I gp120 is capable of interacting with and killing vascular endothelial cells in multiple in vivo contexts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 07:21:44