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Titolo:
Effects of NF-kappa B1 (p50) targeted gene disruption on ionizing radiation-induced NF-kappa-B activation and TNF alpha, IL-1 alpha, IL-1 beta and IL-6 mRNA expression in vivo
Autore:
Zhou, DH; Yu, T; Chen, G; Brown, SA; Yu, ZF; Mattson, MP; Thompson, JS;
Indirizzi:
Univ Kentucky, Sanders Brown Res Ctr Aging, Div Rheumatol Allergy & Immunol, Dept Internal Med, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 nternal Med, Lexington, KY 40536 USA Vet Adm Med Ctr, Lexington, KY 40511 USA Vet Adm Med Ctr Lexington KY USA40511 m Med Ctr, Lexington, KY 40511 USA NIA, Gerontol Res Ctr, Neurosci Lab, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 Res Ctr, Neurosci Lab, Baltimore, MD 21224 USA Med Univ S Carolina, Dept Pathol, Div Res, Charleston, SC 29425 USA Med Univ S Carolina Charleston SC USA 29425 Res, Charleston, SC 29425 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
fascicolo: 7, volume: 77, anno: 2001,
pagine: 763 - 772
SICI:
0955-3002(200107)77:7<763:EONB(T>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; VERSUS-HOST DISEASE; TOTAL-BODY IRRADIATION; MESSENGER-RNA LEVELS; BONE-MARROW TRANSPLANTATION; NORMAL RAT ASTROCYTES; GAMMA-IRRADIATION; INTERLEUKIN-1-BETA GENE; IMMUNE-RESPONSES; MICE LACKING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Zhou, DH Univ Kentucky, Sanders Brown Res Ctr Aging, Div Rheumatol Allergy& Immunol, Dept Internal Med, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 ed, Lexington, KY 40536 USA
Citazione:
D.H. Zhou et al., "Effects of NF-kappa B1 (p50) targeted gene disruption on ionizing radiation-induced NF-kappa-B activation and TNF alpha, IL-1 alpha, IL-1 beta and IL-6 mRNA expression in vivo", INT J RAD B, 77(7), 2001, pp. 763-772

Abstract

Purpose: To investigate the role of the NF-kappaB] (p50) gene in ionizing radiation (IR)-induced NF-kappaB activation and TNF alpha, IL- 1 alpha, IL-1 beta and IL-6 mRNA expression in vivo. Materials and methods: NF-kappaB activation was analysed by the gel shift/supershift assay and the levels or TNF alpha, IL-1 alpha, IL-1 beta and IL-6 mRNA were measured using RNase protection assay (RPA). Various tissues from BALB/c, B6,129P-Nfkb1 (NF-kappa B1 or p50 gene knockout, p50(-/-)) and B6,129PF2 (wild-type, p50(+/+)) mice were analysed before or after exposure to a lethal dose (8.5 Gy) of total-body gamma -irradiation. Results: Exposure of BALB/c mice to total-body IR selectively activated NF-kappaB in the spleen, mesenteric lymph nodes (LN) and bone marrow (BM). Gel supershift assay using polyclonal antibodies against NF-kappaB p50, p65 or c-Rel protein revealed that the NF-kappaB p50 subunit is a critical component of the NF-kappaB complexes activated by IR in vivo. Discretely augmented TNF alpha, IL-1 alpha, IL-1 beta and IL-6 mRNA expression was found in the spleen, LN and BM after BALB/c mice received IR. However, mice lacking the p50 gene (p50(-/-)) showed a significant reduction in IR-induced activation of NF-kappaB and increases in TNF alpha, IL-1 alpha IL-1 beta and IL-6 mRNA expression, as compared with that of wild-type mice (p50(+/+)). Conclusions:The NF-kappaB p50 subunit is a critical component of the NF-kappaB complexes activated by IR and it plays an important role in mediating IR-induced TNF alpha, IL-1 alpha, IL-1 beta and IL-6 mRNA expression in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:41:57