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Titolo:
Increased cytotoxic effects of photodynamic therapy in IL-6 gene transfected cells via enhanced apoptosis
Autore:
Usuda, J; Okunaka, T; Furukawa, K; Tsuchida, T; Kuroiwa, Y; Ohe, Y; Saijo, N; Nishio, K; Konaka, C; Kato, H;
Indirizzi:
Tokyo Med Univ, Dept Surg, Tokyo, Japan Tokyo Med Univ Tokyo JapanTokyo Med Univ, Dept Surg, Tokyo, Japan Tokyo Med Univ, Intractable Dis Res Ctr, Tokyo, Japan Tokyo Med Univ Tokyo Japan Univ, Intractable Dis Res Ctr, Tokyo, Japan Natl Canc Ctr Hosp, Div Med Oncol, Tokyo, Japan Natl Canc Ctr Hosp TokyoJapan nc Ctr Hosp, Div Med Oncol, Tokyo, Japan Natl Canc Ctr, Res Inst, Div Pharmacol, Tokyo 104, Japan Natl Canc Ctr Tokyo Japan 104 Res Inst, Div Pharmacol, Tokyo 104, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 4, volume: 93, anno: 2001,
pagine: 475 - 480
SICI:
0020-7136(20010815)93:4<475:ICEOPT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEWIS LUNG-CARCINOMA; BCL-2; INTERLEUKIN-6; EXPRESSION; ACTIVATION; BAX;
Keywords:
PDT; IL-6; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Usuda, J Case Western Reserve Univ, Sch Med, Dept Radiat Oncol, BRB339,10900 EuclidAve, Cleveland, OH 44106 USA Case Western Reserve Univ BRB339,10900 Euclid Ave Cleveland OH USA 44106
Citazione:
J. Usuda et al., "Increased cytotoxic effects of photodynamic therapy in IL-6 gene transfected cells via enhanced apoptosis", INT J CANC, 93(4), 2001, pp. 475-480

Abstract

PDT has been reported to induce cancer cell expression of cytokines, such as IL-6 and TNF-alpha, but it has been unclear whether cytokine expression by cancer cells is directly related to the antitumor effect of PDT. We treated Lewis lung carcinoma (LLC) cells with a new photosensitizer, mono-L-aspartyl chlorin e6 (NPe6) and light from a diode laser and found that expression of the mRNA of IL-2, IL-6, and TNF-alpha was increased by NPe6-mediated-PDT 6 hr later. To elucidate the mechanism of the direct anti-tumor effectof cytokine expression, we examined the photosensitivity of cytokine-gene-transfected cells, namely LLC-IL-2, LLC-IL-6, and LLC-TNF-alpha cells, by MTT assay. The IL-6 gene transfected, LLC-IL-6 cells were significantly moresensitive to cytotoxic effects than the parent LLC cells and other cytokine gene-transfected cells. This Finding indicates that IL-6 expression modulates cellular sensitivity to PDT and that IL-2 and TNF-alpha expressions does not, In addition, the apoptosis of LLC-IL-6 cells induced by NPe6-PDT was greater than in the other cells as determined by DNA fragmentation and staining of apoptotic nuclei. Because IL-6 has been reported to induce apoptosis by downregulating expression of Bcl-2, we analyzed the expression of apoptosis-related Bcl-2, Bax, and cytochrome C by Western blot analysis. Decreased expression of Bcl-2 and cytochrome C was observed in both LLC calls and LLC-IL-6 cells. Bax protein increased in a time-dependent manner, and the ratio of Bax to Bcl-2 rose markedly after PDT in LLC-IL-6 cells, These results suggest that the increased sensitivity of LLC-IL-6 cells to PDT-induced cytotoxicity results from the high ratio of Bax to Bcl-2 in the IL-6-dependent apoptotic pathway, In conclusion, IL-6 expression plays a role in cellular sensitivity to PDT, and combination of IL-6 and PDT may provide a new strategy for cancer treatment. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:42:53