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Titolo:
Recombinant Dirofilaria immitis-derived antigen can suppress passive cutaneous anaphylaxis reactions
Autore:
Furuhashi, Y; Imai, S; Tezuka, H; Fujita, K;
Indirizzi:
Tokyo Med & Dent Univ, Grad Sch, Div Publ Hlth,Dept Int Hlth Dev, Sect Environm Parasitol,Bunkyo Ku, Tokyo, Japan Tokyo Med & Dent Univ Tokyo Japan onm Parasitol,Bunkyo Ku, Tokyo, Japan
Titolo Testata:
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
fascicolo: 2, volume: 125, anno: 2001,
pagine: 144 - 151
SICI:
1018-2438(200106)125:2<144:RDIACS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NIPPOSTRONGYLUS-BRASILIENSIS INFECTION; EPSILON-RI EXPRESSION; MAST-CELLS; FILARIAL PARASITE; IGE RECEPTORS; IN-VIVO; RATS; MODULATION; RESPONSES; BINDING;
Keywords:
IgE; mast cells; Fc epsilon RI; Dirofilaria immitis; passive cutaneous anaphylaxis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Fujita, K Tokyo Med & Dent Univ, Grad Sch, Div Publ Hlth,Dept Int Hlth Dev, Sect Environm Parasitol,Bunkyo Ku, 1-5-45 Yushima, Tokyo, Japan Tokyo Med& Dent Univ 1-5-45 Yushima Tokyo Japan Tokyo, Japan
Citazione:
Y. Furuhashi et al., "Recombinant Dirofilaria immitis-derived antigen can suppress passive cutaneous anaphylaxis reactions", INT A AL IM, 125(2), 2001, pp. 144-151

Abstract

Background: High levels of antigen-nonspecific IgE are produced in animalsinfected with helminth parasites. Generally, the increase in IgE is thought to exacerbate allergic reactions. However, high levels of antigen-non-specific IgE may alter some features of anaphylactic reactions. To investigatethe molecular mechanisms of antigen-nonspecific IgE production induced during filarial infections, we previously constructed rDiAg (recombinant Dirofilaria immitis-derived antigen) in Escherichia coli. In the present study, we examined the effect of rDiAg on the production of antigen-nonspecific IgE and on allergic cutaneous reactions in rats. Methods: Osmotic pumps filled with 200 mug of rDiAg or with 200 mul of PBS (control) were subcutaneously implanted in Wister rats, and plasma samples were collected weekly thereafter. IgE levels were determined by ELISA. Homologous passive cutaneous anaphylaxis (PCA) reactions with anti-DNP-As IgE were examined 21 days after implantation. I-125-IgE binding assays were examined on peritoneal mast cells from rDiAg-infused rats and control rats. Results: Antigen-nonspecific IgE production was induced in rDiAg-infused rats. PCA reactions were suppressed in rDiAg-infused rats in spite of high levels of IgE and a markedly increased expression of Fc epsilon RI. I-125-IgE did not bind to mast cells derived from rDiAg-infused rats, but it bound dose dependently to mast cells derived from control rats. Conclusion: The present data support the hypothesis that antigen-nonspecific IgE might protect aga inst antigen-specific IgEby means of competition for mast cell receptors. rDiAg is an essential factor to induce antigen-nonspecific IgE in helminth infections. Copyright (C)2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:34:27