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Titolo:
Identification of soluble transforming growth factor-beta receptor III (sTbeta III) in rat milk
Autore:
Zhang, MF; Zola, H; Read, LC; Penttila, IA;
Indirizzi:
Child Hlth Res Inst, Cooperat Res Ctr Tissue Growth & Repair, N Adelaide, SA 5006, Australia Child Hlth Res Inst N Adelaide SA Australia 5006 aide, SA 5006, Australia
Titolo Testata:
IMMUNOLOGY AND CELL BIOLOGY
fascicolo: 3, volume: 79, anno: 2001,
pagine: 291 - 297
SICI:
0818-9641(200106)79:3<291:IOSTGF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
TGF-BETA; PROTEOGLYCAN BETAGLYCAN; RECENT PROGRESS; LIGAND-BINDING; CELLS; EXPRESSION; SYSTEM; PURIFICATION; COMPLEX; FORMS;
Keywords:
maternal milk; suckling intestine; transforming growth factor-beta; transforming growth factor-beta; receptor betaglycan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Penttila, IA Child Hlth Res Inst, Cooperat Res Ctr Tissue Growth & Repair,72 King William Rd, N Adelaide, SA 5006, Australia Child Hlth Res Inst 72 King William Rd N Adelaide SA Australia 5006
Citazione:
M.F. Zhang et al., "Identification of soluble transforming growth factor-beta receptor III (sTbeta III) in rat milk", IMM CELL B, 79(3), 2001, pp. 291-297

Abstract

Transforming growth factor-beta (TGF-beta) is present at high concentrations in maternal milk. In milk TGF-beta2 is the predominant isoform. For function TGF-beta2 requires T beta RIII to facilitate efficient binding to the TGF-beta receptor types I and II signalling complex. We have shown that TGF-beta receptor types I (T beta RI), II (T beta RII) and III (T beta RIII) are coexpressed in the suckling rat intestine. Immunostaining for T beta RIII was also observed in the intestinal lumen prior to weaning. T beta RIII (or betaglycan) has been reported in serum, cell culture medium and extracellular matrix. To determine whether a soluble form of T beta RIII is presentin milk, the rat milk aqueous phase was analysed by slot-blot and Western blot. Soluble T beta RIII was detected in milk throughout lactation. Western blot analysis of rat milk revealed a high molecular weight band of glycosylated protein of >200 kDa, with a core protein of approximately 110-120 kDa that comigrated with recombinant T beta RIII. Immunoabsorption of solubleT beta RIII (sT beta RIII) from milk resulted in partial depletion of active TGF-beta from milk, suggesting that the receptor may interact with ligand in milk. In addition rat pups suckled on mother's milk demonstrated an enhanced labelling of T beta RIII in the gut, as compared with pups fed on a rat milk substitute (RMS). These findings suggest that milk sT beta RIII isfunctional, and may modulate milk-derived TGF-beta function in the developing intestine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 02:12:36