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Titolo:
Generation and characterization of recombinant ScFv antibodies detecting Eimeria acervulina surface antigens
Autore:
Kim, JK; Min, W; Lillehoj, HS; Kim, SW; Sohn, EJ; Song, KD; Han, JY;
Indirizzi:
USDA, Paraite Biol Epidemiol & Systemat Lab, Anim & Nat Resources Inst, Beltsville, MD 20705 USA USDA Beltsville MD USA 20705 Nat Resources Inst, Beltsville, MD 20705 USA Changwon Natl Univ, Dept Microbiol, Changwon, South Korea Changwon Natl Univ Changwon South Korea icrobiol, Changwon, South Korea Seoul Natl Univ, Sch Agr Biotechnol, Suwon, South Korea Seoul Natl Univ Suwon South Korea ch Agr Biotechnol, Suwon, South Korea
Titolo Testata:
HYBRIDOMA
fascicolo: 3, volume: 20, anno: 2001,
pagine: 175 - 181
SICI:
0272-457X(200106)20:3<175:GACORS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICKEN MONOCLONAL-ANTIBODY; MESSENGER-RNA EXPRESSION; PHAGE DISPLAY TECHNOLOGY; TENELLA INFECTION; VARIABLE DOMAINS; T-LYMPHOCYTES; COCCIDIOSIS; GAMMA; GENE; IMMUNIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Lillehoj, HS USDA, Paraite Biol Epidemiol & Systemat Lab, Anim & Nat Resources Inst, Bldg 1040,BARC-E, Beltsville, MD 20705 USA USDA Bldg 1040,BARC-EBeltsville MD USA 20705 e, MD 20705 USA
Citazione:
J.K. Kim et al., "Generation and characterization of recombinant ScFv antibodies detecting Eimeria acervulina surface antigens", HYBRIDOMA, 20(3), 2001, pp. 175-181

Abstract

In our previous attempt to generate monoclonal antibodies (MAbs) against coccidia parasites that more accurately reflect the natural avian humoral immune response, we produced two chicken B-cell hybridomas, 5D11 and 2-1. While both cell Lines secreted antibodies reactive with sporozoites of Eimeriaacervulina, they were produced in yields too low to conduct meaningful in vivo studies, To circumvent this problem, we produced four single chain variable fragment (scFv) antibodies from the VH and VL genes of hybridomas 5D11 and 2-1. The concentration of these recombinant antibodies expressed in E. coil and purified to homogeneity was 5-6 mg/L. Three of the antibodies exhibited antigen binding specificity to Eimeria surface antigens equivalent to that of the native MAbs, Nucleotide sequence analysis of the VL genes from hybridomas 5D11 and 2-1 and genomic DNA revealed vestiges of gene conversion with Vh pseudogenes, These recombinant scFv antibodies will prove useful for further characterization of natural Eimeria surface antigens as potential vaccine candidates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:32:20