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Titolo:
A unique murine monoclonal antibody recognizing HLA-B53, B37, B51, B52, +/-B44
Autore:
Etessami, S; Tarsitani, C; Lias, M; Broadous, M; Conger, N; Randall, G; Delory, M; Bahrami, S; Lee, JH;
Indirizzi:
One Lambda Inc, Canoga Park, CA 91303 USA One Lambda Inc Canoga Park CA USA 91303 da Inc, Canoga Park, CA 91303 USA
Titolo Testata:
HUMAN IMMUNOLOGY
fascicolo: 7, volume: 62, anno: 2001,
pagine: 732 - 738
SICI:
0198-8859(200107)62:7<732:AUMMAR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINGLE AMINO-ACID; PUBLIC EPITOPES; MALARIA; IDENTIFICATION; HLA-B-ASTERISK-4402; ALLOSPECIFICITIES; SUBSTITUTION; PEPTIDES; BINDING; ALLELES;
Keywords:
HLA antigens; HLA-Bw53; malaria; MHC; monoclonal antibody;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Etessami, S One Lambda Inc, Canoga Park, CA 91303 USA One Lambda Inc Canoga Park CA USA 91303 ga Park, CA 91303 USA
Citazione:
S. Etessami et al., "A unique murine monoclonal antibody recognizing HLA-B53, B37, B51, B52, +/-B44", HUMAN IMMUN, 62(7), 2001, pp. 732-738

Abstract

Monoclonal antibodies have played an important role in studying the biochemistry of the HLA-Class I molecules. Some murine anti-HLA mAbs can identifyconfigurations of HLA epitopes that have never been reported in human allosera. One oi these configurations is identified by an IgM mAb designated as: BHA-1441. This antibody was produced using a lymphoblastoid cell line typed as: A*02, A*25; B*38, B*4402/4405; C*0501, C*07, BW4, as the immunogen. A lymphocytotoxicity test of this mAb over a panel of 109 frozen, 452 freshand, later, 44 DNA typed T cells revealed its specificity as B53, 37, 51, 52, +/- 44. All of che antigens recognized by this mAb share the Bw4 motif at positions 81-83, except for the HLA-B37, which shares only 82L and 83R. Furthermore, while B37 and B44 cross-react due to the aspartic acid (D) substitution at position 156, the reactivity with B53, B5 (51,52), B37 and 60%of B44 cells, makes it unlikely that the target epitope could be due only to the primary amino-acid sequence. The antibody-binding site might involvechanges in tertiary structure and peptides bound by the MHC. BHA-1441 is an interesting tool to study and type the HLA-B53 antigen and its cross-reactive epitopes. (C) American Society for Histocompatibility and Immunogenetics, 2001. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 15:57:53