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Titolo:
Synthetic peptides induce a cytotoxic response against human papillomavirus type-18
Autore:
Castellanos, MR; Hayes, RL; Maiman, MA;
Indirizzi:
Staten Isl Univ Hosp, Dept Obstet & Gynecol, Staten Isl, NY 10305 USA Staten Isl Univ Hosp Staten Isl NY USA 10305 ol, Staten Isl, NY 10305 USA Nalitt Inst Canc & Blood Related Dis, Dept Med, Staten Isl, NY USA Nalitt Inst Canc & Blood Related Dis Staten Isl NY USA taten Isl, NY USA Nalitt Inst Canc & Blood Related Dis, Canc Immunotherapy Program, Staten Isl, NY USA Nalitt Inst Canc & Blood Related Dis Staten Isl NY USA taten Isl, NY USA
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 1, volume: 82, anno: 2001,
pagine: 77 - 83
SICI:
0090-8258(200107)82:1<77:SPIACR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CERVICAL-CANCER; PERIPHERAL-BLOOD; T-LYMPHOCYTES; CTL EPITOPES; E6; IDENTIFICATION; NEOPLASIA; MOLECULES; INFECTION; CELLS;
Keywords:
papillomavirus, human; cervical cancer; immunotherapy; vaccine; HLA-A2 antigens; cytotoxic T-lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Castellanos, MR Staten Isl Univ Hosp, Dept Obstet & Gynecol, Staten Isl, NY 10305 USA Staten Isl Univ Hosp Staten Isl NY USA 10305 NY 10305 USA
Citazione:
M.R. Castellanos et al., "Synthetic peptides induce a cytotoxic response against human papillomavirus type-18", GYNECOL ONC, 82(1), 2001, pp. 77-83

Abstract

Objectives. Over 90% of cervical carcinomas express human papillomavirus (HPV) E6 and E7 proteins. These unique antigens are ideal targets for the development of cytotoxic T-lymphocytes (CTL) for antitumor immunotherapy. In this study we identify peptides from HPV-18 E6 and E7 proteins that bind toHLA class I molecules. We further show that these peptides are able to induce peptide-specific CTL from an HLA-A2-positive (+) peripheral blood donorin vitro,Methods. A computer-assisted algorithm was devised to identify peptides from HPV-18 E6 and E7 proteins that bind to HLA-A2 molecules. Peptides that were predicted to bind were synthesized and their binding activity was determined. HLA-A2(+) irradiated stimulator cells pulsed with HPV-18 peptides were incubated with HLA-A2(+) peripheral blood mononuclear cells. Cytotoxicity assays were performed to assess specific cell lysis,Results. Of 295 possible sequences, the computer-assisted algorithm predicted 10 peptides that would have a high probability of binding to HLA-A2. The 4 strongest binding peptides were analyzed for their ability to induce cytotoxic cells against HPV-18 peptide-pulsed targets. Two of the peptides induced significant lysis. Conclusions, There are limited data on peptide-based immunotherapy for HPV-18(+) tumors. The combination of our computer-assisted algorithm and binding assay permits rapid selection of potential CTL epitopes, We identified two peptides that were able to induce peptide-specific lysis. These two epitopes are candidates for a peptide-based vaccine against HPV-18(+) tumors. The model described has broad applications and can be used in the development of immunotherapy for other types of cancers. (C) 2001 Academic Press.

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Documento generato il 08/04/20 alle ore 12:09:51