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Titolo:
An immunomagnetic-based method for the purification of ovarian cancer cells from patient-derived ascites
Autore:
Barker, SD; Casado, E; Gomez-Navarro, J; Xiang, J; Arafat, W; Mahasreshti, P; Pustilnik, TB; Hemminki, A; Siegal, GP; Alvarez, RD; Curiel, DT;
Indirizzi:
Univ Alabama, Div Human Gene Therapy, Gene Therapy Ctr, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 herapy Ctr, Birmingham, AL 35294 USA Univ Alabama, Dept Med, Div Human Genet Therapy, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 et Therapy, Birmingham, AL 35294 USA Univ Alabama, Dept Pathol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ept Pathol, Birmingham, AL 35294 USA Univ Alabama, Dept Surg, Birmingham, AL 35294 USA Univ Alabama BirminghamAL USA 35294 Dept Surg, Birmingham, AL 35294 USA Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Cell Biol, Birmingham, AL 35294 USA Univ Alabama, Dept Obstet & Gynecol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 & Gynecol, Birmingham, AL 35294 USA
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 1, volume: 82, anno: 2001,
pagine: 57 - 63
SICI:
0090-8258(200107)82:1<57:AIMFTP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-CELLS; INTRAPERITONEAL RADIOIMMUNOTHERAPY; MONOCLONAL-ANTIBODY; B72.3; SEPARATION; CARCINOMA; GROWTH; FLUID;
Keywords:
cell separation; immunomagnetic bead technique; Dynabeads; tumor-associated glycoprotein 72; CC49 antibody; ovarian neoplasm;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Curiel, DT Univ Alabama, Div Human Gene Therapy, Gene Therapy Ctr, 1824 6th Ave S,Room WTI 620, Birmingham, AL 35294 USA Univ Alabama 1824 6th Ave S,Room WTI 620 Birmingham AL USA 35294
Citazione:
S.D. Barker et al., "An immunomagnetic-based method for the purification of ovarian cancer cells from patient-derived ascites", GYNECOL ONC, 82(1), 2001, pp. 57-63

Abstract

Objective. Primary ovarian cancer cells obtained from fresh tumor have many advantages over established cell lines, Therefore, a procedure for the specific and efficient purification of such neoplastic cells is critical. We report an effective immunomagnetic method for the isolation of tumor cells from the ascitic fluid of patients diagnosed with ovarian adenocarcinoma,Methods. This procedure incorporates the use of monoclonal antibody (mAb) CC49, which recognizes the tumor-associated glycoprotein 72 (TAG-72), TAG-72 is highly expressed on ovarian tumor cell surfaces with little or no reactivity with normal tissues. Also used in this protocol are immunomagnetic beads, which bind to the CC49 mAb via a secondary antibody. When ovarian cancer cells adhere to the magnetic beads, a magnetic field is used to separate the tumor cells from all other cellular components. Results. Using ascitic fluid from five patients, we found that preparations before purification contained between 38 and 52% neoplastic cells. Using our method, we produced preparations that were between 63 and 96% pure for cancer cells, thus obtaining an average increase in tumor cell enrichment of 86%. Conclusion. We, therefore, believe this method is preferable for producinghigh yields of pure ovarian neoplastic cells. We are now employing this technique in our laboratory to provide a stringent and pure template for our studies on gene transfer to primary ovarian cancer cells. (C) 2001 AcademicPress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:19:00