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Titolo:
Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1 -> 4GlcNAc beta 1 -> R in both N- and O-glycans
Autore:
Hiraoka, N; Misra, A; Belot, F; Hindsgaul, O; Fukuda, M;
Indirizzi:
Burnham Inst, Canc Res Ctr, Glycobiol Program, La Jolla, CA 92037 USA Burnham Inst La Jolla CA USA 92037 cobiol Program, La Jolla, CA 92037 USA
Titolo Testata:
GLYCOBIOLOGY
fascicolo: 6, volume: 11, anno: 2001,
pagine: 495 - 504
SICI:
0959-6658(200106)11:6<495:MCAEOT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASPARAGINE-LINKED OLIGOSACCHARIDES; HEPARAN-SULFATE; L-SELECTIN; BOVINE PROOPIOMELANOCORTIN; CHORIONIC-GONADOTROPIN; STRUCTURAL ELUCIDATION; HNK-1 SULFOTRANSFERASE; GLYCOPROTEIN; GALNAC; LUTROPIN;
Keywords:
GalNAc 4-O-sulfotransferase; pituitary hormone glycoproteins; mucin-type O-glycans; HNK-1 sulfotransferase gene family;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Fukuda, M Burnham Inst, Canc Res Ctr, Glycobiol Program, 10901 N Torrey Pines Rd, LaJolla, CA 92037 USA Burnham Inst 10901 N Torrey Pines Rd La JollaCA USA 92037 7 USA
Citazione:
N. Hiraoka et al., "Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1 -> 4GlcNAc beta 1 -> R in both N- and O-glycans", GLYCOBIOLOG, 11(6), 2001, pp. 495-504

Abstract

Recently, cDNAs encoding human chondroitin 4-O-sulfotransferase-1 and -2 (C4ST-1 and C4ST-2) were cloned based on their similarity to HNK-1 sulfotransferase (HNK-1ST) (Hiraoka, N., Nakagawa, H., Ong, E., Akama, T,O., Fukuda,M,N., and Fukuda, M. [2000] Molecular cloning and expression of two distinct human chondroitin 4-O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family. J. Biol. Chem., 275, 20188-20196), In the present study, we identified two additional novel sulfotransferases by searching the expression sequence tag and genomic DNA database for enzymes similar to C4ST-1 and C4ST-2. These newly cloned enzymes, termed GalNAc4ST-1 and GalNAc4ST-2, belong to the HNK-1ST gene family having 40-42% identity with C4ST-1. GalNAc4ST-1 and -2 do not add sulfate to HNK-1 precursor glycans, chondroitin, or desulfated dermatan sulfate. Instead, both enzymes can transfer sulfate to the 4-position of GalNAc in the context of GalNAc-beta1 --> 4GlcNAc beta1 -->R attached to both N-linked and core 2 branched O-linked oligosaccharides. GalNAc4ST-1 and -2 transcripts are highly expressed in the pituitary gland and trachea, respectively, and GalNAc4ST-1 and -2 transcripts are reciprocally expressed in other tissues as well, Moreover, both enzymes cantransfer sulfate to lutropin, a pituitary glycoprotein hormone, These combined results indicate that GalNAc4ST-1 and -2 play critical roles in forming sulfo --> 4GalNAc beta1 --> 4GlcNAc beta1 -->R in both N-glycans and O-glycans in a tissue-specific manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:23:03