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Titolo:
Retinol binding protein in rainbow trout: Molecular properties and mRNA expression in tissues
Autore:
Sammar, M; Babin, PJ; Durliat, M; Meiri, I; Zchori, I; Elizur, A; Lubzens, E;
Indirizzi:
Israel Oceanog & Limnol Res, IL-31080 Haifa, Israel Israel Oceanog & Limnol Res Haifa Israel IL-31080 IL-31080 Haifa, Israel Univ Bordeaux 1, USC INRA, F-33405 Talence, France Univ Bordeaux 1 Talence France F-33405 USC INRA, F-33405 Talence, France Univ Paris Sud, UPRESA 8080 CNRS, F-91405 Orsay, France Univ Paris Sud Orsay France F-91405 ESA 8080 CNRS, F-91405 Orsay, France Israel Oceanog & Limnol Res, Eilat, Israel Israel Oceanog & Limnol Res Eilat Israel og & Limnol Res, Eilat, Israel Tel Aviv Univ, Dept Zool, Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel el Aviv Univ, Dept Zool, Tel Aviv, Israel
Titolo Testata:
GENERAL AND COMPARATIVE ENDOCRINOLOGY
fascicolo: 1, volume: 123, anno: 2001,
pagine: 51 - 61
SICI:
0016-6480(200107)123:1<51:RBPIRT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITELLOGENIN MESSENGER-RNA; VITAMIN-A; ONCORHYNCHUS-MYKISS; 3-DIMENSIONAL STRUCTURE; VERTEBRATE DEVELOPMENT; ESTROGEN INDUCTION; PLASMA; SERUM; TRANSTHYRETIN; TRANSPORT;
Keywords:
retinol-binding protein; cDNA; trout; vitellogenin; liver; 17 beta-estradiol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Lubzens, E Israel Oceanog & Limnol Res, IL-31080 Haifa, Israel Israel Oceanog & Limnol Res Haifa Israel IL-31080 ifa, Israel
Citazione:
M. Sammar et al., "Retinol binding protein in rainbow trout: Molecular properties and mRNA expression in tissues", GEN C ENDOC, 123(1), 2001, pp. 51-61

Abstract

Retinoids are important regulatory signaling molecules during embryonic development. The molecular properties of rainbow trout (Oncorhynchus mykiss) retinol-binding protein (rtRBP), the specific retinol carrier in vertebrateplasma, were studied to elucidate its role in transporting retinols to developing fish oocytes. A 954-nucleotide rtRBP cDNA was cloned from the livercoding for a 176-amino-acid (aa) mature protein, with an estimated molecular mass of 20,267 Da. The nucleotide sequence suggests a putative 16-aa signal peptide and shows all the aa residues that were previously identified as critical for the retinol binding pocket. Five of the eight amino acid residues that are associated with the interaction of REP and transthyretin in mammalian and nonmammalian species are conserved. The deduced aa sequence of rtRBP shows 60 - 66% identity with zebrafish, chicken, mouse, rat, horse,bovine, and human RBPs and 56% identity with Xenopus REP. Northern blot analysis revealed a similar to1.1-kb hepatic mRNA transcript. REP is highly expressed in the liver, but low levels were also detected in the spleen, kidney, ovary, and brain. In the rainbow trout, 17 beta -estradiol treatment led to a decrease in the REP mRNA signal relative to that of the controls. The efficacy of the 17 beta -estradiol treatment was verified by an induction of vitellogenin (VTG) mRNA expression in the liver and occurrence of VTG in the plasma. (C) 2001 Academic Press.

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Documento generato il 04/04/20 alle ore 07:50:28