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Titolo:
Adaptable doxycycline-regulated gene expression systems for Drosophila
Autore:
Stebbins, MJ; Yin, JCP;
Indirizzi:
Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA Cold Spring Harbor Lab Cold Spring Harbor NY USA 11724 rbor, NY 11724 USA SUNY Stony Brook, Genet Program, Stony Brook, NY 11790 USA SUNY Stony Brook Stony Brook NY USA 11790 gram, Stony Brook, NY 11790 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 270, anno: 2001,
pagine: 103 - 111
SICI:
0378-1119(20010530)270:1-2<103:ADGESF>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN-CELLS; HEAT-SHOCK; DOMINANT; MEMORY; BOUNDARIES; ASSAY;
Keywords:
inducible; transgenic; spatial/temporal; tetracycline; Tet-Off transactivator;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Yin, JCP Cold Spring Harbor Lab, 1 Bungtown Rd, Cold Spring Harbor, NY 11724 USA Cold Spring Harbor Lab 1 Bungtown Rd Cold Spring Harbor NY USA 11724
Citazione:
M.J. Stebbins e J.C.P. Yin, "Adaptable doxycycline-regulated gene expression systems for Drosophila", GENE, 270(1-2), 2001, pp. 103-111

Abstract

We have engineered two new versions of the doxycycline (dox) inducible system for use in Drosophila. In the first system, we have used the ubiquitously expressed Drosophila actin5C promoter to express the Tet-Off transactivator (tTA) in all tissue. Induction of a luciferase target transgene begins 6 h after placing the flies on dox-free food. Feeding drug-free food to mothers results in universal target gene expression in their embryos. Larvae raised on regular food also show robust expression of a target reporter gene. In the second version, we have used the Gal4-UAS system to spatially limit expression of the transactivator. Dox withdrawal results in temporally- and spatially restricted, inducible expression of luciferase in the adult head and embryo. Both the actin5C and Gal4-UAS versions produce more than 100-fold induction of luciferase in the adult, with virtually no leaky expression in the presence of drug. Reporter gene expression is also undetectable in larvae or embryos from mothers fed dox-containing food. Such tight control may be due to the incorporation of Drosophila insulator elements (SCS and SCS ') into the transgenic vectors. These systems offer a practical, effective alternative to currently available expression systems in the Drosophila research community. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 17:36:18