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Titolo:
Plasmodium falciparum: A major role for IgG3 in antibody-dependent monocyte-mediated cellular inhibition of parasite growth in vitro
Autore:
Tebo, AE; Kremsner, PG; Luty, AJF;
Indirizzi:
Univ Tubingen, Inst Trop Med, Dept Parasitol, D-72074 Tubingen, Germany Univ Tubingen Tubingen Germany D-72074 asitol, D-72074 Tubingen, Germany Albert Schweitzer Hosp, Res Unit, Lambarene, Gabon Albert Schweitzer HospLambarene Gabon Hosp, Res Unit, Lambarene, Gabon
Titolo Testata:
EXPERIMENTAL PARASITOLOGY
fascicolo: 1, volume: 98, anno: 2001,
pagine: 20 - 28
SICI:
0014-4894(200105)98:1<20:PFAMRF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMATE-RICH PROTEIN; MEROZOITE SURFACE PROTEIN-1; BLOOD-STAGE ANTIGENS; IMMUNOGLOBULIN-G; IN-VITRO; PROTECTIVE IMMUNITY; MALARIA INFECTION; GAMBIAN CHILDREN; MECHANISMS; RESPONSES;
Keywords:
apicomplexia; malaria; antibody isotypes; growth inhibition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Luty, AJF Univ Tubingen, Inst Trop Med, Dept Parasitol, Wilhelmstr 27, D-72074 Tubingen, Germany Univ Tubingen Wilhelmstr 27 Tubingen Germany D-72074 n, Germany
Citazione:
A.E. Tebo et al., "Plasmodium falciparum: A major role for IgG3 in antibody-dependent monocyte-mediated cellular inhibition of parasite growth in vitro", EXP PARASIT, 98(1), 2001, pp. 20-28

Abstract

In an attempt to identify parasite antigen-specific antibody isotype(s) mediating inhibition of growth in wine, we tested unfractionated sera and their corresponding purified antibody isotype-containing fractions in in vitroassays with asexual-stage parasites of Plasmodium falciparum in the presence or absence of monocytes. Using affinity purification techniques, we fractionated individual and pooled serum samples from semi-immune Gabonese adults, to obtain samples containing either IgG1, 2. 3, and 4, IgG1, 2, and 4, or IgG3 alone, and a non-IgG fraction. Antibodies were quantified spectrophotometrically and the presence of different isotypes in individual fractions was confirmed by protein gel electrophoresis. In the absence of monocytes, we observed inhibition of parasite growth with whole serum and varying levels of either growth enhancement or inhibition with purified Ig-containingfractions. When used in a standardized assay of antibody-dependent cellular inhibition (ADCI) with a monocyte:infected erythrocyte ratio of 1:1, seven of eight serum samples Inhibited growth to a mean level of 42%, and the different Ig-containing fractions displayed varying mean levels of inhibition: IgG3, 44%; IgG;1-4, 22%; IgG1, 2, and 4, 10%; and non-IgG, -10%. The results suggest that, among the different isotypes present in the serum of semi-immune individuals, parasite antigen-specific IgG3 in particular may playan important role in controlling parasitemia via an ADCI mechanism involving monocyte-derived mediators, (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:05:28