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Titolo:
Effects of type 2 cytokines on glomerular epithelial cells
Autore:
Parry, RG; Gillespie, KM; Mathieson, PW;
Indirizzi:
Southmead Hosp, Acad Renal Unit, Bristol, Avon, England Southmead Hosp Bristol Avon England d Renal Unit, Bristol, Avon, England
Titolo Testata:
EXPERIMENTAL NEPHROLOGY
fascicolo: 4, volume: 9, anno: 2001,
pagine: 275 - 283
SICI:
1018-7782(2001)9:4<275:EOT2CO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; IDIOPATHIC NEPHROTIC SYNDROME; PERMEABILITY FACTOR RELEASE; MESSENGER-RNA EXPRESSION; BLOOD MONONUCLEAR-CELLS; RECEPTOR-ALPHA CHAIN; EXPERIMENTAL GLOMERULONEPHRITIS; CRESCENTIC GLOMERULONEPHRITIS; INTERLEUKIN-10 RECEPTOR; GENE-EXPRESSION;
Keywords:
cytokines; cytokine receptors; glomerular epithelial cells; vascular endothelial growth factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Parry, RG Royal Devon & Exeter Hosp Wonford, Renal Unit, Exeter EX2 5DW, Devon, England Royal Devon & Exeter Hosp Wonford Exeter Devon England EX2 5DW
Citazione:
R.G. Parry et al., "Effects of type 2 cytokines on glomerular epithelial cells", EXP NEPHROL, 9(4), 2001, pp. 275-283

Abstract

Visceral glomerular epithelial cells (GECs) are involved in the maintenance of the filtration barrier and may play a role in immune responses. Cytokines may act on GECs and we wished to test this in vitro. Vascular endothelial growth factor (VEGF) is a specific product of the GEC that may play a role in glomerular permeability. We have investigated whether GECs in cultureexpress receptors for interleukin (IL)-4, 10 and 13 (often grouped together as type 2 cytokines) and whether these cytokines alter GEC VEGF production. Type 2 cytokines were compared to transforming growth factor-beta (TGF-beta) and IL-1 beta which are known to upregulate VEGF production. GECs weregrown from human nephrectomy specimens and cultured with and without the addition of exogenous cytokines. Messenger RNA data demonstrated the presence of IL-4 receptor alpha, IL-10 receptor 1 and 2, and IL-13 receptors alpha(1) and alpha (2). However, at the protein level by flow cytometry, only IL-13 alpha (2) could be consistently demonstrated. IL-4, IL-10 and IL-13 inhibited production of VEGF but did not affect the pattern of isoform expression. In contrast, TBF-beta and IL-1 beta caused an increase in VEGF production. These effects were not explained by effects on proliferation. Our data provide evidence that GECs express receptors for type 2 cytokines and that these cytokines can act directly on GECs, to decrease VEGF production. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:23:06