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Titolo:
Oligodendroglial tau filament formation in transgenic mice expressing G272V tau
Autore:
Gotz, J; Tolnay, M; Barmettler, R; Chen, F; Probst, A; Nitsch, RM;
Indirizzi:
Univ Zurich, Div Psychiat Res, CH-8008 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8008 Res, CH-8008 Zurich, Switzerland Univ Basel, Dept Neuropathol, CH-4003 Basel, Switzerland Univ Basel Basel Switzerland CH-4003 opathol, CH-4003 Basel, Switzerland
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 11, volume: 13, anno: 2001,
pagine: 2131 - 2140
SICI:
0953-816X(200106)13:11<2131:OTFFIT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE SYSTEM ATROPHY; PROGRESSIVE SUPRANUCLEAR PALSY; INHERITED DEMENTIA FTDP-17; SUBUNIT C-ALPHA; ALZHEIMERS-DISEASE; SOMATODENDRITIC LOCALIZATION; NEURODEGENERATIVE DISEASES; CORTICOBASAL DEGENERATION; NEUROFIBRILLARY TANGLES; FRONTOTEMPORAL DEMENTIA;
Keywords:
Alzheimer's disease; frontotemporal dementia; phosphorylation; transactivator;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Gotz, J Univ Zurich, Div Psychiat Res, August Forel Str 1, CH-8008 Zurich,Switzerland Univ Zurich August Forel Str 1 Zurich Switzerland CH-8008 zerland
Citazione:
J. Gotz et al., "Oligodendroglial tau filament formation in transgenic mice expressing G272V tau", EUR J NEURO, 13(11), 2001, pp. 2131-2140

Abstract

Genetic evidence indicates that several mutations in tau, including G272V,are linked to frontotemporal dementia with parkinsonism. We expressed thismutation in mouse brains by combining a prion protein promoter-driven expression system with an autoregulatory transactivator loop that resulted in high expression of human G272V tau in neurons and in oligodendrocytes. We show that G272V tau can form filaments in murine oligodendrocytes. Electron microscopy established that the filaments were either straight or had a twisted structure; these were 17-20 nm wide and had a periodicity of approximate to 75 nm, Filament formation was associated with tau phosphorylation at distinct sites, including the AT8 epitope 202/205 in vivo. Immunogold electron microscopy of sarcosyl-extracted spinal cords from G272V transgenic miceusing phosphorylation-dependent antibodies AT8 or AT100 identified severalsparsely gold-labelled 6-nm filaments. In the spinal cord, fibrillary inclusions were also identified by thioflavin-S fluorescent microscopy in oligodendrocytes and motor neurons. These results establish that expression of the G272V mutation in mice causes oligodendroglial fibrillary lesions that are similar to those seen in human tauopathies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:36:28