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Titolo:
Induction of strong hepatitis B virus (HBV) specific T helper cell and cytotoxic T lymphocyte responses by therapeutic vaccination in the trimera mouse model of chronic HBV infection
Autore:
Bocher, WO; Dekel, B; Schwerin, W; Geissler, M; Hoffmann, S; Rohwer, A; Arditti, F; Cooper, A; Bernhard, H; Berrebi, A; Rose-John, S; Shaul, Y; Galle, PR; Lohr, HF; Reisner, Y;
Indirizzi:
Univ Hosp, Dept Internal Med 1, D-55131 Mainz, Germany Univ Hosp Mainz Germany D-55131 t Internal Med 1, D-55131 Mainz, Germany Weizmann Inst Sci, Dept Immunol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 unol, IL-76100 Rehovot, Israel Univ Hosp, Dept Med 2, Freiburg, Germany Univ Hosp Freiburg GermanyUniv Hosp, Dept Med 2, Freiburg, Germany Weizmann Inst Sci, Dept Virol, IL-76100 Rehovot, Israel Weizmann Inst SciRehovot Israel IL-76100 irol, IL-76100 Rehovot, Israel Tech Univ, Dept Internal Med 3, Munich, Germany Tech Univ Munich Germany ech Univ, Dept Internal Med 3, Munich, Germany Kaplan Hosp, Dept Haematol, IL-76100 Rehovot, Israel Kaplan Hosp RehovotIsrael IL-76100 t Haematol, IL-76100 Rehovot, Israel Univ Kiel, Dept Biochem, Kiel, Germany Univ Kiel Kiel GermanyUniv Kiel, Dept Biochem, Kiel, Germany
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 7, volume: 31, anno: 2001,
pagine: 2071 - 2079
SICI:
0014-2980(200107)31:7<2071:IOSHBV>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PERIPHERAL-BLOOD; SEVERE COMBINED IMMUNODEFICIENCY; HEMATOPOIETIC PROGENITOR CELLS; HUMAN/MOUSE RADIATION CHIMERA; IMMUNE-RESPONSES; DENDRITIC CELLS; SCID MICE; SURFACE-ANTIGEN; IN-VITRO; MONONUCLEAR-CELLS;
Keywords:
hepatitis B virus; immunotherapy; trimera mouse model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Bocher, WO Univ Hosp, Dept Internal Med 1, Langenbeckstr 1, D-55131 Mainz,Germany Univ Hosp Langenbeckstr 1 Mainz Germany D-55131 Mainz, Germany
Citazione:
W.O. Bocher et al., "Induction of strong hepatitis B virus (HBV) specific T helper cell and cytotoxic T lymphocyte responses by therapeutic vaccination in the trimera mouse model of chronic HBV infection", EUR J IMMUN, 31(7), 2001, pp. 2071-2079

Abstract

Humanized BALB/c mice (termed trimera mice) conditioned by lethal total body irradiation and bone marrow transplantation from SCID mice have been described to support rapid engraftment of human peripheral blood mononuclear cells (PBMC) and the induction of strong B and T cell responses after immunization in vivo. Moreover, these mice can be infected with the hepatitis B and C viruses (HBV, HCV). The current study employed this model to study therapeutic vaccination approaches against the HBV. Thus, strong primary Th cell responses against the HBV core (HBc) and the Borrelia burgdorferi control antigen were induced by transfer of antigen-loaded dendritic cells together with autologous PBMC from HBV-naive donors as well as by vaccination with high doses of antigen or a DNA plasmid encoding for HBcAg. Moreover, primary peptide-specific CTL responses against the immunodominant epitope HBc(18-27) were induced by HBc particle or DNA vaccination of chimera engrafted with HBV-naive PBMC. Finally, strong HBc-specific Th cell and antibody responses were induced by HBc or DNA vaccination of mice reconstituted with PBMC from a chronic HBV patient. Thus, since HBc represents the immunodominantantigen in self-limited HBV infection, HBc particles or DNA vectors are good candidates for therapeutic vaccination, that will be further studied in our model and clinical studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:15:03