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Titolo:
Isolation and characterisation of cDNAs encoding the large and small subunits of ADP-glucose pyrophosphorylase from cassava (Manihot esculenta Crantz)
Autore:
Munyikwa, TRI; Kreuze, J; Fregene, M; Suurs, L; Jacobsen, E; Visser, RGF;
Indirizzi:
Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands Univ Wageningen & Res Ctr Wageningen Netherlands NL-6700 AJ Netherlands CIAT, Biotechnol Unit, Cali, Colombia CIAT Cali ColombiaCIAT, Biotechnol Unit, Cali, Colombia
Titolo Testata:
EUPHYTICA
fascicolo: 1, volume: 120, anno: 2001,
pagine: 71 - 83
SICI:
0014-2336(2001)120:1<71:IACOCE>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOUND STARCH SYNTHASE; ADPGLUCOSE PYROPHOSPHORYLASE; MOLECULAR CHARACTERIZATION; ARABIDOPSIS-THALIANA; MULTIPLE FORMS; POTATO; EXPRESSION; CLONING; MAIZE; ENDOSPERM;
Keywords:
AGPase B; AGPase S; ADP-glucose pyrophosphorylase; antisense; cassava; potato;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Visser, RGF Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Lab Plant Breeding, POB 386, NL-6700 AJ Wageningen, Netherlands Univ Wageningen & ResCtr POB 386 Wageningen Netherlands NL-6700 AJ
Citazione:
T.R.I. Munyikwa et al., "Isolation and characterisation of cDNAs encoding the large and small subunits of ADP-glucose pyrophosphorylase from cassava (Manihot esculenta Crantz)", EUPHYTICA, 120(1), 2001, pp. 71-83

Abstract

Screening of a tuber specific cassava cDNA library resulted in the isolation of full length cDNA clones with homology to the genes encoding the smalland large subunits of ADP glucose pyrophosphoryalse. Sequence analysis revealed that AGPase B the clone with homology to the small subunit shared 54%homology at amino acid level with the AGPase S clone that is more closely related to the large subunit. Segregation analysis of a cross between the cassava cultivars TMS 30572 and CM 2177-2 revealed that AGPase S is a singlecopy gene that is localised on the female derived linkage group E of the cassava genetic map. AGPase B is a low copy gene of which one member is localised on the female derived linkage group P. The two genes are expressed inall cassava tissues but AGPase B exhibits a higher steady state mRNA levelthan AGPase S and is highly expressed in leaf and tuber tissue. The AGPaseenzyme activity was much higher in young cassava leaves as compared to older leaves and tubers. Cassava AGPase was activated by 3-PGA and inhibited by up to 90% in the presence of inorganic phosphate (Pi). The tuber enzyme was relatively unaffected by 3PGA but was highly inhibited by Pi. Transformation of potato (Solanum tuberosum) plants with an antisense AGPase B construct resulted in 10 out of 134 antisense AGPase B plants having on average 3.5 times more tubers than the control non transgenic plants. Analysis of these transgenic plants revealed they had greatly reduced levels of AGPase B mRNA, 1.5 to 3 times less starch, and five times higher levels of soluble sugars, sucrose, glucose and fructose, to those found in control plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 16:59:15