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Titolo:
In vivo modulation of nonylphenol-induced zonagenesis and vitellogenesis by the antiestrogen, 3,3 ' 4,4 '-tetrachlorobiphenyl (PCB-77) in juvenile fish
Autore:
Arukwe, A; Yadetie, F; Male, R; Goksoyr, A;
Indirizzi:
Univ Bergen, HIB, Dept Mol Biol, N-5020 Bergen, Norway Univ Bergen Bergen Norway N-5020 B, Dept Mol Biol, N-5020 Bergen, Norway
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
fascicolo: 1-2, volume: 10, anno: 2001,
pagine: 5 - 15
SICI:
1382-6689(200105/06)10:1-2<5:IVMONZ>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALMON SALMO-SALAR; COD GADUS-MORHUA; ALKYLPHENOL POLYETHOXYLATE SURFACTANTS; ANNUAL REPRODUCTIVE-CYCLE; MYKISS LIVER-CELLS; ESTROGEN-RECEPTOR; AQUATIC ENVIRONMENT; BETA-NAPHTHOFLAVONE; GENE-EXPRESSION; PLASMA-LEVELS;
Keywords:
zonagenesis; vitellogenesis; 3,3 ',4,4 '-tetrachlorobiphenyl (TCB);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Arukwe, A Univ Bergen, HIB, Dept Mol Biol, POB 7800, N-5020 Bergen, NorwayUniv Bergen POB 7800 Bergen Norway N-5020 N-5020 Bergen, Norway
Citazione:
A. Arukwe et al., "In vivo modulation of nonylphenol-induced zonagenesis and vitellogenesis by the antiestrogen, 3,3 ' 4,4 '-tetrachlorobiphenyl (PCB-77) in juvenile fish", ENV TOX PH, 10(1-2), 2001, pp. 5-15

Abstract

Zonagenesis and vitellogenesis (eggshell zona radiata protein (Zrp) and vitellogenin (Vtg) production, respectively), are two estrogen-regulated processes in oviparous vertebrates that are crucial for oocyte maturation. Treatment of juvenile Atlantic salmon (Salmo salar) with nonylphenol (NP; 25 mgkg(-1)) alone or in combination with 3,3',4,4'-tetrachlorobiphenyl (TCB; 0.1 mg kg(-1)) resulted in pronounced elevations of plasma eggshell Zrp and Vtg and their respective liver mRNA levels in two separate experiments. TCBtreatment alone caused the elevation of CYP1A mRNA, protein and enzyme levels (7-ethoxyresorufin O-deethylase (EROD)). In experiment 3, which also included the time factor, exposure of juvenile salmon to 10 and 25 mg NP per kg in combination with TCB generally resulted in reduced plasma Zrp and Vtglevels, compared with NP treatments alone. In a fourth experiment, juvenile salmon were exposed to different doses of TCB either 2 days before or 2 days after a single dose (25 mg kg(-1)) of NP. Samples were always collected5 days after the NP exposure and analyzed for mRNA and protein levels. Generally, TCB doses given 2 days after NP exposure resulted in the elevation of Vtg and Zrp protein and mRNA levels. Vtg and Zrp mRNA. levels were also elevated in the groups treated with 0.1 mg TCB 2 days before NP exposure. In all experiments, TCB injection resulted in the induction of liver CYP1A mRNA, CYP1A protein and EROD activity: but no Zrp or Vtg protein/mRNA inductions were observed when given alone. The present study documents for the first time the apparent stimulation of xenoestrogen-induced responses by an antiestrogenic CYP1A-inducer, in fish or any other lower vertebrate. However, the stimulatory or inhibitory effect of TCB on NP-induced responses appear to be dependent on the ratio of NP and TCB doses, and temporal sequence of exposure. Fish hepatic zonagenesis and vitellogenesis continue to provideinteresting models for further studies on the mechanisms and possible interactions between endocrine disrupters and CYP1A-inducers, their antiestrogenic and/or estrogen potentiating effects. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 02:29:50