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Titolo:
Analysis of Kudoa thyrsites (Myxozoa : Myxosporea) spore antigens using monoclonal antibodies
Autore:
Chase, JC; Dawson-Coates, JA; Haddow, JD; Stewart, MH; Haines, LR; Whitaker, DJ; Kent, ML; Olafson, RW; Pearson, TW;
Indirizzi:
Univ Victoria, Dept Biochem & Microbiol, Victoria, BC V8W 3P6, Canada UnivVictoria Victoria BC Canada V8W 3P6 ol, Victoria, BC V8W 3P6, Canada Fisheries & Oceans Canada, Pacific Biol Stn, Nanaimo, BC V9R 5K6, Canada Fisheries & Oceans Canada Nanaimo BC Canada V9R 5K6 o, BC V9R 5K6, Canada
Titolo Testata:
DISEASES OF AQUATIC ORGANISMS
fascicolo: 2, volume: 45, anno: 2001,
pagine: 121 - 129
SICI:
0177-5103(20010620)45:2<121:AOKT(:>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AQUACULTURE; FISHERIES; LIGHT;
Keywords:
Atlantic salmon; Salmo salar; Kudoa thyrsites; multivalvulida; marine myxosporean; soft flesh syndrome; plasmodia; spores; monoclonal antibodies; immunodetection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Pearson, TW Univ Victoria, Dept Biochem & Microbiol, Petch Bldg, Victoria,BC V8W 3P6,Canada Univ Victoria Petch Bldg Victoria BC Canada V8W 3P6 3P6,Canada
Citazione:
J.C. Chase et al., "Analysis of Kudoa thyrsites (Myxozoa : Myxosporea) spore antigens using monoclonal antibodies", DIS AQU ORG, 45(2), 2001, pp. 121-129

Abstract

A method employing Percoll (TM) gradient centrifugation was developed to purify Kudoa thyrsites spores from somatic muscle tissue of Atlantic salmon Salmo salar. Highly purified spores were then used to immunize inbred BALB/c mice for derivation of hybridomas secreting Kudoa-specific monoclonal antibodies (mAbs). Analysis of mAbs by immunofluorescence microscopy and flow cytometry showed that several were specific for antigens on the surface of K, thyrsites spores whereas other mAbs reacted with polar capsules or with polar filaments of spores of K, thyrsites, K. paniformis and K. crumena. Immunoblots on spore lysates using the surface-binding mAbs showed a broad band of 46 to > 220 kDa, whereas mAbs specific for antigens of polar capsulesand polar filaments detected sharper bands of various molecular masses, depending on the Kudoa species. The dominant epitope of the K, thyrsites spore surface antigen was shown to be carbohydrate as determined by its sensitivity to treatment with anhydrous trifluoromethane sulfonic acid and by its resistance to treatment with Proteinase K. Immunofluorescence microscopy using the K. thyrsites-specific mAbs on isolated, intact, permeabilized plasmodia and on thin sections of somatic muscle tissue containing plasmodia revealed intense labeling of spores both within the spore-producing plasmodia and in the flesh of infected Atlantic salmon. As few as 100 spores were detected by immunoblotting, indicating that these mAbs have potential for use in developing a field-based diagnostic test.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:54:57