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Titolo:
Development of a ceuE-based multiplex polymerase chain reaction (PCR) assay for direct detection and differentiation of Campylobacter jejuni and Campylobacter coli in Thailand
Autore:
Houng, HSH; Sethabutr, O; Nirdnoy, W; Katz, DE; Pang, LW;
Indirizzi:
Walter Reed Army Inst Res, Dept Enter Infect, Silver Spring, MD 20910 USA Walter Reed Army Inst Res Silver Spring MD USA 20910 Spring, MD 20910 USA Armed Forces Res Inst Med Sci, USA, Med Component, Dept Enter Dis, Bangkok10400, Thailand Armed Forces Res Inst Med Sci Bangkok Thailand 10400 gkok10400, Thailand
Titolo Testata:
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
fascicolo: 1-2, volume: 40, anno: 2001,
pagine: 11 - 19
SICI:
0732-8893(200105/06)40:1-2<11:DOACMP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROINVASIVE ESCHERICHIA-COLI; RESISTANCE; AMPLIFICATION; SHIGELLA; SEQUENCE; FECES;
Keywords:
Campylobacter jejuni; Campylobacter coli; polymerase chain reaction (PCR); ceuE locus; hippurate hydrolysis; quinolone antibiotics resistance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Houng, HSH Walter Reed Army Inst Res, Dept Virus Dis, Silver Spring, MD 20910 USA Walter Reed Army Inst Res Silver Spring MD USA 20910 20910 USA
Citazione:
H.S.H. Houng et al., "Development of a ceuE-based multiplex polymerase chain reaction (PCR) assay for direct detection and differentiation of Campylobacter jejuni and Campylobacter coli in Thailand", DIAG MICR I, 40(1-2), 2001, pp. 11-19

Abstract

A novel ceuE-based multiplex PCR system was developed as an efficient diagnostics test to detect and differentiate C. jejuni and C, coil. There is nocross reactivity between C. jejuni and C. coli. In addition, the assay does not produce a positive signal from other enteric bacteria including Salmonella, Shigella and Escherichia coli strains. Campylobacter detection sensitivity was determined to be equivalent to previously reported PCR for otherenteric bacteria. We also noticed that silicon dioxide extraction can improve Campylobacter detection sensitivity from infected stool samples. It wasdemonstrated that the PCR assay developed in this study had a much better Campylobacter detection rate than the traditional culturing method (77% versus 56%). However, we also identified small numbers of culture positive stools (8%, or 16 out of 202 samples) that did not yield PCR positive results for Campylobacter. These PCR negative/culture positive stools were proven to be inhibitory to PCR amplification. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 10:11:36