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Titolo:
The homeobox genes vox and vent are redundant repressors of dorsal fates in zebrafish
Autore:
Imai, Y; Gates, MA; Melby, AE; Kimelman, D; Schier, AF; Talbot, WS;
Indirizzi:
Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Dept Dev Biol, Stanford, CA 94305 USA Univ Washington, Dept Biochem, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Biochem, Seattle, WA 98195 USA NYU, Sch Med, Skirball Inst Biomol Med, Dept Cell Bio,Dev Genet Program, New York, NY 10016 USA NYU New York NY USA 10016 l Bio,Dev Genet Program, New York, NY 10016 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 12, volume: 128, anno: 2001,
pagine: 2407 - 2420
SICI:
0950-1991(200106)128:12<2407:THGVAV>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DORSOVENTRAL PATTERN-FORMATION; EARLY XENOPUS EMBRYOS; BETA-CATENIN; ORGANIZER; INDUCTION; MESODERM; EXPRESSION; XVENT-2; BMP-4; REQUIREMENT;
Keywords:
vox; vent; bozozok; chordin; goosecoid; BMP; gastrulation; morpholino; zebrafish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Talbot, WS Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ol, Stanford, CA 94305 USA
Citazione:
Y. Imai et al., "The homeobox genes vox and vent are redundant repressors of dorsal fates in zebrafish", DEVELOPMENT, 128(12), 2001, pp. 2407-2420

Abstract

Ventralizing transcriptional repressors in the Vox/Vent family have been proposed to be important regulators of dorsoventral patterning in the early embryo, While the zebrafish genes vox (vega1) and vent (vega2) both have ventralizing activity in overexpression assays, loss-of function studies are needed to determine whether these genes have distinct or redundant functions in dorsoventral patterning and to provide critical tests of the proposed regulatory interactions among vex, vent and other genes that act to establish the dorsoventral axis. We show that vox and vent are redundant repressors of dorsal fates in zebrafish. Mutants that lack vox function have little or no dorsoventral patterning defect, and inactivation of either vox or vent by injection of antisense morpholino oligonucleotides has little or no effect on the embryo. In contrast, embryos that lack both vox and vent function have a dorsalized phenotype, Expression of dorsal mesodermal genes, including chordin, goosecoid and bozozok, is strongly expanded in embryos that lack vox and vent function, indicating that the redundant action of vox andvent is required to restrict dorsal genes to their appropriate territories, Our genetic analysis indicates that the dorsalizing transcription factor Bozozok promotes dorsal fates indirectly, by antagonizing the expression ofvox and vent. In turn, vox and vent repress chordin expression, restricting its function as an antagonist of ventral fates to the dorsal side of the embryo, Our results support a model in which BMP signaling induces the expression of ventral genes, while vox and vent act redundantly to prevent the expression of chordin, goosecoid and other dorsal genes in the lateral and ventral mesendoderm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:06:08