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Titolo:
Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity
Autore:
Chen, WB; Burgess, S; Hopkins, N;
Indirizzi:
MIT, Ctr Canc Res, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139MIT, Ctr Canc Res, Cambridge, MA 02139 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 12, volume: 128, anno: 2001,
pagine: 2385 - 2396
SICI:
0950-1991(200106)128:12<2385:AOTZSM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOTOR-NEURON INDUCTION; CENTRAL-NERVOUS-SYSTEM; VENTRAL CELL-TYPES; PROTEIN-KINASE-A; SONIC-HEDGEHOG; FLOOR PLATE; SIGNAL-TRANSDUCTION; DOPAMINERGIC-NEURONS; SPINAL-CORD; GENE;
Keywords:
zebrafish; hedgehog; smoothened; floor plate; motoneuron; dopaminergic neurons; left-right asymmetry; apoptosis; cell proliferation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
81
Recensione:
Indirizzi per estratti:
Indirizzo: Hopkins, N MIT, Ctr Canc Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA MIT 77 Massachusetts Ave Cambridge MA USA 02139 e, MA 02139 USA
Citazione:
W.B. Chen et al., "Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity", DEVELOPMENT, 128(12), 2001, pp. 2385-2396

Abstract

Despite extensive studies, there are still many unanswered questions regarding the mechanism of hedgehog signaling and the phylogenic conservation ofhedgehog function in vertebrates. For example, whether hedgehog signaling in vertebrates requires smoothened is unclear, and the role of hedgehog activity in zebrafish is controversial. We show that inactivation of smoothened by retroviral insertions in zebrafish results in defects that are characteristic of hedgehog deficiencies, including abnormalities in body size, thecentral nervous system, adaxial mesoderm, cartilage and pectoral fins, We demonstrate that, as in Drosophila, vertebrate smoothened is essential for hedgehog signaling, and functions upstream of protein kinase A, Further analysis of neural tube defects revealed the absence of lateral floor plate and secondary motoneurons, but the presence of medial floor plate and primarymotoneurons in smoothened mutant embryos. Blocking maternal hedgehog signaling by cyclopamine eliminates primary motoneurons, but not medial floor plate. Interestingly, even after inhibition of maternal hedgehog activity, the midbrain dopaminergic neurons still form, and looping of the heart does not randomize in the mutants. We also found decreased proliferation and increased apoptosis in the mutants, Taken together, these data demonstrate the conserved role of vertebrate smoothened in the hedgehog signaling pathway, and reveal similarities and differences of hedgehog function between teleosts and amniotes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:15:10