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Titolo:
Pre-pattern in the pronephric kidney field of zebrafish
Autore:
Serluca, FC; Fishman, MC;
Indirizzi:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Cardiovasc Res Ctr, Boston,MA 02129 USA Harvard Univ Boston MA USA 02129 Cardiovasc Res Ctr, Boston,MA 02129 USA Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dev Biol Lab, Boston, MA 02129 USA Harvard Univ Boston MA USA 02129 Med, Dev Biol Lab, Boston, MA 02129 USA Harvard Univ, Sch Med, Dept Med, Boston, MA 02129 USA Harvard Univ BostonMA USA 02129 Sch Med, Dept Med, Boston, MA 02129 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 12, volume: 128, anno: 2001,
pagine: 2233 - 2241
SICI:
0950-1991(200106)128:12<2233:PITPKF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILMS-TUMOR GENE; XENOPUS-LAEVIS; EXPRESSION; HEART; DUCT; LINEAGE; SYSTEM; CELLS; DIFFERENTIATION; SPECIFICATION;
Keywords:
pronephros; kidney field; glomerulus; cell fate; regulative development; zebrafish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Fishman, MC Harvard Univ, Massachusetts Gen Hosp, Sch Med, Cardiovasc Res Ctr, Boston,MA 02129 USA Harvard Univ Boston MA USA 02129 Res Ctr, Boston,MA 02129 USA
Citazione:
F.C. Serluca e M.C. Fishman, "Pre-pattern in the pronephric kidney field of zebrafish", DEVELOPMENT, 128(12), 2001, pp. 2233-2241

Abstract

Vertebrate embryos use a series of transient kidneys to regulate fluid balance, osmolarity and metabolic waste during development. The first kidney to form in the embryo is the pronephros. This kidney is composed of several cell types with very different functions and is organized into discrete segments: glomerulus, tubules and nephric duct. The site of origin of these cells is poorly understood, as are their lineage relationships. We have defined regions of the intermediate mesoderm as candidates for the pronephric field by expression patterns of the Wilms' Tumor suppressor gene (wt1), single-minded 1 (sim1) and pax2.1, All of these potential kidney markers are expressed in a stripe of intermediate mesoderm, with distinct, overlapping antero-posterior borders. We labeled small groups of cells in this area by laser uncaging of a fluorescent dextran, and then tracked their fates. We found that there was a bounded contiguous region of the intermediate mesoderm that provides pronephric progenitors. As is true for other organ fields, thepronephric field regulates after focal destruction, such that a normal pronephros forms after laser-mediated removal of the wt1 domain, The progenitors for podocytes, tubular cells and duct are restricted to subdomains within the pronephric field. The most anterior cells in the pronephric field give rise to podocytes, This corresponds to the wt1-expressing region. The next more posterior cells contribute to the tubule, and express both wt1 and pax2.1. The most posterior cells contribute to the nephric duct, and these express pax2.1 and sim1, but not wt1. Thus, there is a field for the pronephric kidney with classical attributes of defined border, pre-pattern and regulation. The pattern of the fate map reflects particular combinations of transcription factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 00:59:35