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Titolo:
Three-dimensional morphological analysis of antigen-antibody reaction in hepatic sinusoids preserved in hypothermic UW solution
Autore:
Sasaki, K; Johkura, K; Ogiwara, N; Liang, Y; Cui, L; Teng, R; Okouchi, Y; Asanuma, K; Tshida, O; Maruyama, K;
Indirizzi:
Shinshu Univ, Sch Med, Dept Anat & Organ Technol, Matsumoto, Nagano 3908621, Japan Shinshu Univ Matsumoto Nagano Japan 3908621 sumoto, Nagano 3908621, Japan Shinshu Univ, Grad Sch Med, Inst Organ Transplants Reconstruct Med & Tissue E, Matsumoto, Nagano 3908621, Japan Shinshu Univ Matsumoto Nagano Japan 3908621 sumoto, Nagano 3908621, Japan Teikyo Univ, Fac Pharmaceut Sci, Kanagawa 1990195, Japan Teikyo Univ Kanagawa Japan 1990195 armaceut Sci, Kanagawa 1990195, Japan
Titolo Testata:
CRYOBIOLOGY
fascicolo: 2, volume: 42, anno: 2001,
pagine: 145 - 150
SICI:
0011-2240(200103)42:2<145:TMAOAR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER; CELLS; CYTOSKELETON; REPERFUSION; MOUSE;
Keywords:
UW solution; antigen-antibody reaction; ICAM-1; immunoliposome; immunoSEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Sasaki, K Shinshu Univ, Sch Med, Dept Anat & Organ Technol, 3-1-1 Asahi, Matsumoto, Nagano 3908621, Japan Shinshu Univ 3-1-1 Asahi Matsumoto Nagano Japan 3908621 1, Japan
Citazione:
K. Sasaki et al., "Three-dimensional morphological analysis of antigen-antibody reaction in hepatic sinusoids preserved in hypothermic UW solution", CRYOBIOLOGY, 42(2), 2001, pp. 145-150

Abstract

ICAM-1 antigen-antibody reaction was visualized by three-dimensional immunoscanning electron microscopy of hepatic sinusoids in rat liver treated with hypothermic University of Wisconsin (UW) organ preservation solution. Theresults were compared with similar antigen-antibody reactions carried out with immunoliposomes injected in vivo. Morphologically, the hepatic sinusoids were preserved well during the hypothermic procedure. Endothelial cells had a large number of fenestrations, which partly aggregated and formed sieve plates. ICAM-1 expression was induced by injection of LPS and detected by monoclonal antibody in the UW solution followed by gold-labeled secondaryantibody. ICAM-1 was restricted mostly to the unique areas of sieve plateswith immature, small fenestrations. A similar distribution of ICAM-1 was present when detected by in vivo injection of immunoliposomes containing themonoclonal ICAM-1 antibody. The results showed that antigen-antibody reactions can take place in livers preserved in hypothermic UW solution. Further, the reaction is similar to that which could occur in vivo during transplantation. This suggests that it may be possible to block potentially harmfulantigen-antibody reactions by addition of appropriate antibodies to hypothermic UW solution prior to transplantation. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 22:04:02