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Titolo:
Polycolonization of Helicobacter pylori among Chinese subjects
Autore:
Yakoob, J; Fan, XG; Hu, GL; Yang, HX; Liu, L; Liu, SH; Tan, DM; Li, TG; Zhang, Z;
Indirizzi:
Hunan Med Univ, Xiangya Hosp, Dept Infect Dis, Changsha, Hunan, Peoples R China Hunan Med Univ Changsha Hunan Peoples R China ha, Hunan, Peoples R China Hunan Med Univ, Xiangya Hosp, Dept Gastroenterol, Changsha, Hunan, PeoplesR China Hunan Med Univ Changsha Hunan Peoples R China sha, Hunan, PeoplesR China First Hosp Changsha, Dept Gastroenterol, Changsha, Hunan, Peoples R China First Hosp Changsha Changsha Hunan Peoples R China unan, Peoples R China
Titolo Testata:
CLINICAL MICROBIOLOGY AND INFECTION
fascicolo: 4, volume: 7, anno: 2001,
pagine: 187 - 192
SICI:
1198-743X(200104)7:4<187:POHPAC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
GASTRIC ANTRUM; DIVERSITY; INFECTION; PATTERNS; STRAINS; SINGLE;
Keywords:
Helicobacter pylori; DNA heterogeneity; antibiotic susceptibility test; multiple strain colonization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Yakoob, J Def Housing Soc, 1-B 7th Cent Lane,Phase 2, Karachi, Pakistan Def Housing Soc 1-B 7th Cent Lane,Phase 2 Karachi Pakistan tan
Citazione:
J. Yakoob et al., "Polycolonization of Helicobacter pylori among Chinese subjects", CL MICRO IN, 7(4), 2001, pp. 187-192

Abstract

Objective To investigate the situation among Chinese patients with regard to infection with multiple strains of Helicobacter pylori. Methods Biopsy specimens for culture of H. pylori were obtained from gastric antrum, body and fundus of 20 patients during endoscopic investigation of upper gastrointestinal symptoms. H. pylori was identified by culture fromone site in 16 and two or more sites in 10 of the 16 patients. Five isolated colonies of six strains of H. pylori from gastric antrum were subcultured and used for further analysis. Antibiotic susceptibility to metronidazoleand clarithromycin was determined by disk diffusion rest. Protein profilesof isolates were compared by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). DNA diversity of the isolates was determined by arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting. Results Of the 10 patients with multiple isolates, 70% (7/10) exhibited variation in susceptibility to metronidazole and 20% (2/10) to clarithromycinbetween different sites. In 83% of (5/6) single colonies, no variability was seen in metronidazole and clarithromycin susceptibility; they were either susceptible or resistant. Protein profiles of all isolates by SDS-PAGE were similar. Isolates from different patients produced clearly different AP-PCR fingerprints. In 50% of H. pylori strains isolated front different sites of the stomach, genetic diversity was demonstrated by different AP-PCR fingerprints. In 67% (4/6) strains, five single-colony fingerprints were similar. Conclusions Genetic variability has ben found in H. pylori strains. Individual patients are infected with a single predominant genotype at a single site bur can be colonized by multiple strains, and they may show different antibiotic susceptibilities. Individual colonies of the H. pylori populationfront a single site may not always yield identical DNA fingerprints and antibiotic sensitivities.

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Documento generato il 01/12/20 alle ore 10:02:05