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Titolo:
Reliability and validity of commercially available, direct radioimmunoassays for measurement of blood androgens and estrogens in postmenopausal women
Autore:
Rinaldi, S; Dechaud, H; Biessy, C; Morin-Raverot, V; Toniolo, P; Zeleniuch-Jacquotte, A; Akhmedkhanov, A; Shore, RE; Secreto, G; Ciampi, A; Riboli, E; Kaaks, R;
Indirizzi:
Int Agcy Res Canc, Unit Nutr Canc, F-69372 Lyon, France Int Agcy Res CancLyon France F-69372 it Nutr Canc, F-69372 Lyon, France Hop Antiquaille, Cent Lab Biochem, F-69005 Lyon, France Hop Antiquaille Lyon France F-69005 nt Lab Biochem, F-69005 Lyon, France NYU, Sch Med, Dept Obstet & Gynecol, New York, NY 10010 USA NYU New York NY USA 10010 , Dept Obstet & Gynecol, New York, NY 10010 USA NYU, Sch Med, Dept Environm Med, New York, NY 10010 USA NYU New York NY USA 10010 Med, Dept Environm Med, New York, NY 10010 USA Natl Canc Inst, Unit Endocrinol, I-20133 Milan, Italy Natl Canc Inst Milan Italy I-20133 Unit Endocrinol, I-20133 Milan, Italy McGill Univ, Dept Epidemiol & Biostat, Montreal, PQ H3A 1A2, Canada McGillUniv Montreal PQ Canada H3A 1A2 stat, Montreal, PQ H3A 1A2, Canada
Titolo Testata:
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
fascicolo: 7, volume: 10, anno: 2001,
pagine: 757 - 765
SICI:
1055-9965(200107)10:7<757:RAVOCA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE-BINDING GLOBULIN; BREAST-CANCER; ESTRADIOL MEASUREMENT; REPLACEMENT THERAPY; SERUM ESTRADIOL; BONE LOSS; TESTOSTERONE; REPRODUCIBILITY; ASSAYS; RISK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kaaks, R Int Agcy Res Canc, Unit Nutr Canc, 150 Cours Albert Thomas, F-69372 Lyon, France Int Agcy Res Canc 150 Cours Albert Thomas Lyon France F-69372 ce
Citazione:
S. Rinaldi et al., "Reliability and validity of commercially available, direct radioimmunoassays for measurement of blood androgens and estrogens in postmenopausal women", CANC EPID B, 10(7), 2001, pp. 757-765

Abstract

In large-scale epidemiological studies on endogenous sex steroids and cancer risk, direct immunoassays of circulating hormone levels have the advantage of being fast and comparatively inexpensive while requiring only small sample volumes. On the other hand, indirect assays after organic extraction and chromatographic prepurification have the advantage of reducing specificinterferences and matrix effects and hence are thought to have better validity. We compared direct assays of testosterone (T, six different assays), Delta4-androstenedione (A, four assays), estrone (E-1, one assay), and 17 beta -estradiol (E-2, five assays) with measurements obtained by an indirectassay in a representative subset of 20 postmenopausal women who were part of a large prospective cohort study. Within-batch reproducibilities of the subject rankings by relative hormonelevels were good (intraclass correlations >0.89) for all direct assays tested. Between batches, reproducibilities generally were also acceptable (r >0.80) to good (r > 0.90) in terms of Pearson's correlations. The between-batch reproducibility in terms of intraclass correlations was systematicallylower in terms of Pearson's correlations, however, because of between-batch variations in the absolute scale of measurements. The relative validity of direct versus indirect assays in terms of the subjects' ranking by relative hormone levels was also high for most of the kits tested for T, A, and E-1 (Pearson's correlations between 0.70 and 0.89) but was high for only twokits of five tested for E-2 (correlations of 0.86 and 0.84). On an absolute scale, mean measurement values were generally higher for direct assays than for the indirect assay and, for each hormone, varied substantially, depending on the kit used. Overall, the results of this study show that, with careful selection, commercial kits for direct radioimmunoassays of steroid hormones in postmenopausal serum can be found that may allow a reliable estimation of relative risks in epidemiological studies. However, standardization of the absolute scale of assays remains problematic.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:22:10