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Titolo:
Short-term tumor cell lines from breast cancer for use as autologous tumorcell vaccines in the treatment of breast cancer
Autore:
Dillman, RO; Beutel, LD; De Leon, C; Nayak, SK;
Indirizzi:
Hoag Canc Ctr, Newport Beach, CA 92658 USA Hoag Canc Ctr Newport Beach CAUSA 92658 Ctr, Newport Beach, CA 92658 USA
Titolo Testata:
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
fascicolo: 3, volume: 16, anno: 2001,
pagine: 205 - 211
SICI:
1084-9785(200106)16:3<205:STCLFB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
OVARIAN-CANCER; MEDICAL PROGRESS; IMMUNOTHERAPY; CARCINOMA; STRATEGIES; CULTURES; ANTIBODY; THERAPY; PROTEIN; GENE;
Keywords:
breast cancer; vaccines; tumor cell cultures; tumor cell lines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Dillman, RO Hoag Canc Ctr, 1 Hoag Dr,Bldg 41, Newport Beach, CA 92658 USA Hoag Canc Ctr 1 Hoag Dr,Bldg 41 Newport Beach CA USA 92658 USA
Citazione:
R.O. Dillman et al., "Short-term tumor cell lines from breast cancer for use as autologous tumorcell vaccines in the treatment of breast cancer", CANC BIO R, 16(3), 2001, pp. 205-211

Abstract

Objective: We tried to establish short-term cultures of autologous tumors from patients with breast carcinoma for potential use as active specific immunotherapy (i.e., autologous vaccine) after resection of primary breast cancer, and/or for the treatment of metastases. Methods: Between 10/90 and 12/99 the cell biology laboratory of the Hoag Cancer Center attempted to establish short-term tumor cell lines from 115 breast cancer specimens from 56 primary breast lesions, 17 axillary nodes, 14 other lymph node/soft tissue sites, 10 chest wall recurrences, and 6 thoracenteses of malignant pleural effusions. Success was defined by growth of 5 X 10(7) viable cells whose malignant nature and breast cancer origin was confirmed by histology of the submitted tissue, cell morphology and antigenic phenotyping. Variables associated with successful growth of short-term cell lines were examined Results: Expansion to 5 X 10(7) cells was achieved for only 8/115 samples [7%] including two from chest wall recurrences, and one each from a supraclavicularnode, an umbilical node, liver, omentum, and pleural fluid. Two of the successful cell lines were established from tissue that originally had been cryopreserved; the others were initiated from fresh tumor. The success rate was better from regional/distant metastases 7/55 (13%) compared to primary tumors 1/56 (1.8%) (p = 0.063). The success rate for tumors harvested at Hoag Hospital was 4/97 (4%) compared to 4/14 from (31%) distant sites, but allbut one of the tumors from a distant geographic site was a metastatic lesion. Tumor cell lines were successfully established from metastatic lesions ranging in size from <1.0 g to 19 g. Four patients were treated with their autologous vaccine in the setting of chemotherapy-refractory metastatic disease without any significant toxicity. Conclusions: We were unable to establish short-term cell lines for most patients with primary or metastatic breast cancer using this methodology. However, two long-term cell lines have been established and characterized Treatment M dth the autologous irradiatedcell product was not associated with acute toxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:50:24