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Titolo:
Identification of myelodysplastic syndrome-specific genes by DNA microarray analysis with purified hematopoietic stem cell fraction
Autore:
Miyazato, A; Ueno, S; Ohmine, K; Ueda, M; Yoshida, K; Yamashita, Y; Kaneko, T; Mori, M; Kirito, K; Toshima, M; Nakamura, Y; Saito, K; Kano, Y; Furusawa, S; Ozawa, K; Mano, H;
Indirizzi:
Jichi Med Sch, Div Funct Genom, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Div Hematol, Minami Kawachi, Tochigi, Japan Jichi Med Sch Minami Kawachi Tochigi Japan inami Kawachi, Tochigi, Japan Jichi Med Sch, Div Cardiol, Minami Kawachi, Tochigi, Japan Jichi Med Sch Minami Kawachi Tochigi Japan inami Kawachi, Tochigi, Japan Jichi Med Sch, Div Mol Immunol, Minami Kawachi, Tochigi, Japan Jichi Med Sch Minami Kawachi Tochigi Japan inami Kawachi, Tochigi, Japan Dokkyo Univ, Sch Med, Dept Hematol, Mibu, Tochigi 32102, Japan Dokkyo Univ Mibu Tochigi Japan 32102 Hematol, Mibu, Tochigi 32102, Japan Tochigi Canc Ctr, Utsunomiya, Tochigi, Japan Tochigi Canc Ctr Utsunomiya Tochigi Japan tr, Utsunomiya, Tochigi, Japan
Titolo Testata:
BLOOD
fascicolo: 2, volume: 98, anno: 2001,
pagine: 422 - 427
SICI:
0006-4971(20010715)98:2<422:IOMSGB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYELOID-LEUKEMIA; CDNA MICROARRAYS; MOLECULAR-CLONING; LEPTIN RECEPTOR; EXPRESSION; PROTEIN; DLK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Mano, H Jichi Med Sch, Div Funct Genom, 3311-1 Yakushiji, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Minami Kawachi Tochigi Japan 3290498
Citazione:
A. Miyazato et al., "Identification of myelodysplastic syndrome-specific genes by DNA microarray analysis with purified hematopoietic stem cell fraction", BLOOD, 98(2), 2001, pp. 422-427

Abstract

Myelodysplastic syndrome (MDS) is a slowly progressing hematologic malignancy associated with a poor outcome. Despite the relatively high incidence of MDS in the elderly differentiation of MDS from de novo acute myeloid leukemia (AML) still remains problematic. Identification of genes expressed in an MDS-specific manner would allow the molecular diagnosis of MDS. Toward this goal, AC133 surface marker-positive hematopoietic stem cell (HSC)-like fractions have been collected from a variety of leukemias in a large-scale and long-term genomics project, referred to as "Blast Bank," and transcriptome of these purified blasts from the patients with MDS were then compared with those from AML through the use of oligonucleotide microarrays. A number of genes were shown to he expressed in a disease-specific manner either to MDS or AML. Among the former found was the gene encoding the protein Delta-like (Dlk) that is distantly related to the Delta-Notch family of signaling proteins. Because overexpression of Dlk may play a role in the pathogenesis of MDS, the disease specificity of Dlk-expression was tested by a quantitative "realtime" polymerase chain reaction analysis. Examination of the Blast Bank samples from 22 patients with MDS, 31 with AML, and 8 with chronic myeloid leukemia confirmed the highly selective expression of the Dlk gene in the individuals with MDS. Dlk could be the first candidate molecule todifferentiate MDS from AML. The proposal is made that microarray analysis with the Blast Bank samples is an efficient approach to extract transcriptome data of clinical relevance for a wide range of hematologic disorders. (C) 2001 by The American Society of Hematology.

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Documento generato il 05/04/20 alle ore 00:52:17