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Titolo:
General strategy for constructing large HSV-1 plasmid vectors that co-express multiple genes
Autore:
Wang, XD; Zhang, GR; Sun, M; Geller, AL;
Indirizzi:
Childrens Hosp, Div Endocrinol, Boston, MA 02115 USA Childrens Hosp Boston MA USA 02115 , Div Endocrinol, Boston, MA 02115 USA Harvard Univ, Sch Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 vard Univ, Sch Med, Boston, MA 02115 USA
Titolo Testata:
BIOTECHNIQUES
fascicolo: 1, volume: 31, anno: 2001,
pagine: 204 -
SICI:
0736-6205(200107)31:1<204:GSFCLH>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMPLEX VIRUS TYPE-1; PROTEIN-KINASE-C; TYROSINE-HYDROXYLASE; CELLS; DECARBOXYLASE; CASSETTE; MUTANT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Geller, AL Childrens Hosp, Div Endocrinol, 300 Longwood Ave, Boston, MA 02115 USA Childrens Hosp 300 Longwood Ave Boston MA USA 02115 A 02115 USA
Citazione:
X.D. Wang et al., "General strategy for constructing large HSV-1 plasmid vectors that co-express multiple genes", BIOTECHNIQU, 31(1), 2001, pp. 204

Abstract

Herpes simplex vir us type 1 (HSV-1) plasmid vectors have a number of attractive features for gene transfer into neurons. In particular the large size of the HSV-1 genome suggests that HSV-1 vectors might be designed to co-express multiple genes. Here, we report a general strategy for constructing large HSV-1 plasmid vectors that co-express multiple genes. Each transcription unit is linked to an antibiotic resistance gene, and genetic selectionsare used to assemble large vectors. Using this strategy, we constructed large (26 or 31 kb) HSV-1 vectors that contain two transcription units and two or three genes. These vectors were efficiently packaged into HSV-1 pat-tides using a helper virus-free packaging system. The resulting vector stockssupported the expression of two or three genes in both cultured cells and the rat brain. Potential applications of HSV-1 vectors that co-express multiple genes are discussed.

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Documento generato il 25/01/20 alle ore 09:32:49