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Titolo:
Fluorescence in situ hybridization method for co-localization of mRNA and GFP
Autore:
Oliva, AA; Swann, JW;
Indirizzi:
Oregon Hlth Sci Univ, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 i Univ, Portland, OR 97201 USA Baylor Coll Med, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ylor Coll Med, Houston, TX 77030 USA
Titolo Testata:
BIOTECHNIQUES
fascicolo: 1, volume: 31, anno: 2001,
pagine: 74 -
SICI:
0736-6205(200107)31:1<74:FISHMF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMIC-ACID DECARBOXYLASE; ENCODING 2 FORMS; MESSENGER-RNAS; INTERNEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Swann, JW Cain Fdn Labs, 6621 Fannin St,MC 3-6365, Houston, TX 77030 USA Cain Fdn Labs 6621 Fannin St,MC 3-6365 Houston TX USA 77030 USA
Citazione:
A.A. Oliva e J.W. Swann, "Fluorescence in situ hybridization method for co-localization of mRNA and GFP", BIOTECHNIQU, 31(1), 2001, pp. 74

Abstract

Co-localization studies using green fluorescent (GFP) and fluorescence immunohistochemistry have become commonplace. However, co-localization studiesusing GFP and nRNA in situ hybridization are rare, in large part because typical in situ hybridization reaction conditions often lead to the loss of GFP fluorescence. Here, we describe a new fluorescence mRNA in situ hybridization protocol using cRNA riboprobes that leaves GFP fluorescence intact. This protocol is based on a urea-based hybridization buffer and the Tyramide Signal Amplification (TM) system. This protocol should provide researchers engaged in the use of GFP with a solid starting point for adapting their own in situ hybridization protocols.

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Documento generato il 25/01/20 alle ore 06:49:13