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Titolo:
Simultaneous determination of clozapine, norclozapine and clozapine-N-oxide in human plasma by high-performance liquid chromatography with ultraviolet detection
Autore:
Liu, YY; van Troostwijk, LJAED; Guchelaar, HJ;
Indirizzi:
Univ Amsterdam, Acad Med Ctr, Pharmaceut & Clin Toxicol Lab EO127, Dept Pharm, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ Z Amsterdam, Netherlands
Titolo Testata:
BIOMEDICAL CHROMATOGRAPHY
fascicolo: 4, volume: 15, anno: 2001,
pagine: 280 - 286
SICI:
0269-3879(200106)15:4<280:SDOCNA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; HUMAN-SERUM; MAJOR METABOLITES; DESMETHYLCLOZAPINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Guchelaar, HJ Univ Amsterdam, Acad Med Ctr, Pharmaceut & Clin Toxicol Lab EO127, Dept Pharm, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Meibergdreef 9 Amsterdam Netherlands NL-1105 AZ
Citazione:
Y.Y. Liu et al., "Simultaneous determination of clozapine, norclozapine and clozapine-N-oxide in human plasma by high-performance liquid chromatography with ultraviolet detection", BIOMED CHRO, 15(4), 2001, pp. 280-286

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method forthe simultaneous determination of clozapine and its two major metabolites,norclozapine and clozapine-N-oxide in human plasma has been developed and validated. The isocratic HPLC assay uses a mobile phase consisting of an acetonitril-buffered aqueous solution containing 146 muL of triethylamine and200 muL of 85% phosphoric acid, adjusted to pH 3.3 with 10% potassiumhydroxide solution (400:600, v/v) at a flow-rate of 0.8 ml/min and a Lichrospher100 RP-18 reversed-phase column and UV detection at 215 nm. Doxepine was used as the internal standard. Mean recoveries for clozapine, norclozapine, clozapine-N-oxide and doxepine were 95%, 98%, 96% and 94% respectively, whereas the respective mean repeatability coefficients of variation were 3.4%,2.7%, 4.3% and 0.9%. Reproducibility coefficients of variation were 1.3%, 1.8%, 3.6% and 0.5%, respectively. The mean correlation coefficient for thelinear calibration curve (n = 2) for clozapine and norclozapine at a concentration range of 100-1600 ng/mL was 0.9998 and 0.9997, respectively; for clozapine-N-oxide (20-200 ng/mL) it was found to be 0.9986, The lower limitsof quantitation were 12.5 ng/mL, 10 ng/mL and 12.5 ng/mL for clozapine, norclozapine and clozapine-N-oxide, respectively. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:47:32