Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The nematode Caenorhabditis elegans synthesizes unusual O-linked glycans: identification of glucose-substituted mucin-type O-glycans and short chondroitin-like oligosaccharides
Autore:
Guerardel, Y; Balanzino, L; Maes, E; Leroy, Y; Coddeville, B; Oriol, R; Strecker, G;
Indirizzi:
Univ Sci & Technol Lille, Chim Biol Lab, F-59655 Villeneuve Dascq, France Univ Sci & Technol Lille Villeneuve Dascq France F-59655 e Dascq, France Univ Sci & Technol Lille, Unite Mixte Rech, CNRS 8576, F-59655 Villeneuve Dascq, France Univ Sci & Technol Lille Villeneuve Dascq France F-59655 e Dascq, France Univ Paris Sud 11, INSERM, U 504, Villejuif, France Univ Paris Sud 11 Villejuif France 11, INSERM, U 504, Villejuif, France
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 357, anno: 2001,
parte:, 1
pagine: 167 - 182
SICI:
0264-6021(20010701)357:<167:TNCESU>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTROSCOPY; GAS-LIQUID-CHROMATOGRAPHY; CANIS INFECTIVE LARVAE; PROTEIN LINKAGE REGION; STRUCTURAL-ANALYSIS; TOXOCARA-CANIS; FUCOSYL-TRANSFERASE; TRYPANOSOMA-CRUZI; METHYL-GLYCOSIDES; N-GLYCANS;
Keywords:
glycosaminoglycan; mass spectrometry; NMR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Guerardel, Y Univ Sci & Technol Lille, Chim Biol Lab, F-59655 Villeneuve Dascq, France Univ Sci & Technol Lille Villeneuve Dascq France F-59655 ce
Citazione:
Y. Guerardel et al., "The nematode Caenorhabditis elegans synthesizes unusual O-linked glycans: identification of glucose-substituted mucin-type O-glycans and short chondroitin-like oligosaccharides", BIOCHEM J, 357, 2001, pp. 167-182

Abstract

The free-living nematode Caenorhabditis elegans is a relevant model for studies on the role of glycoconjugates during development of multicellular organisms. Several genes coding for glycosyltransferases involved in the synthesis of N- and O-linked glycans have already been isolated, but, apart from repetitive dimers of glycosaminoglycans, no detailed structure of either type of component has been published so far. This study aimed to establish the structures of the major O-glycans synthesized by C. Elegans to give an insight into the endogenous glycosyltransferase activities expressed in this organism. By the use of NMR and MS, we have resolved the sequence of seven of these components that present very unusual features, Most of them werecharacterized by the type-1 core substituted on Gal and/or GalNAc by (beta1-4)Glc and (beta1-6)Glc residues. Another compound exhibited the GalNAc(beta1-4)N-acetylglucosaminitol sequence in the terminal position, to which was attached a tetramer of beta -Gal substituted by both Fuc and 2-O-methyl-fucose residues. Our experimental procedure led also to the isolation of glycosaminoglycan-like components acid oligomannosyl-type N-glycans. In particular, the data confirmed that C. elegans synthesizes the ubiquitous linker sequence GlcA(beta1-3)Gal(beta1-3)Gal(beta1-4)Xyl.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:36:08