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Titolo:
Time sequence of the inhibition of endothelial adhesion molecule expression by reconstituted high density lipoproteins
Autore:
Clay, MA; Pyle, DH; Rye, KA; Vadas, MA; Gamble, JR; Barter, PJ;
Indirizzi:
Univ Adelaide, Dept Med, Royal Adelaide Hosp, Adelaide, SA 5000, AustraliaUniv Adelaide Adelaide SA Australia 5000 sp, Adelaide, SA 5000, Australia Inst Med & Vet Sci, Hanson Ctr Canc Res, Adelaide, SA 5000, Australia InstMed & Vet Sci Adelaide SA Australia 5000 delaide, SA 5000, Australia Royal Adelaide Hosp, Cardiovasc Invest Unit, Adelaide, SA 5000, Australia Royal Adelaide Hosp Adelaide SA Australia 5000 elaide, SA 5000, Australia
Titolo Testata:
ATHEROSCLEROSIS
fascicolo: 1, volume: 157, anno: 2001,
pagine: 23 - 29
SICI:
0021-9150(200107)157:1<23:TSOTIO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOKINE-INDUCED EXPRESSION; CORONARY HEART-DISEASE; A-I; VCAM-1 EXPRESSION; CELLS; HDL; APOLIPOPROTEINS; ATHEROSCLEROSIS; MICE; CHOLESTEROL;
Keywords:
reconstituted HDL; endothelial cells; VCAM-1; time sequence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Barter, PJ Univ Adelaide, Dept Med, Royal Adelaide Hosp, N Terrace, Adelaide, SA 5000, Australia Univ Adelaide N Terrace Adelaide SA Australia 5000 0, Australia
Citazione:
M.A. Clay et al., "Time sequence of the inhibition of endothelial adhesion molecule expression by reconstituted high density lipoproteins", ATHEROSCLER, 157(1), 2001, pp. 23-29

Abstract

We have used discoidal reconstituted high density lipoproteins (rHDL) containing apolipoprotein (apo) A-I and dimyristoyl phosphatidylcholine (DMPC) as a tool to investigate the time sequence of the HDL-mediated inhibition of vascular cell adhesion molecule (VCAM)-1 and E-selectin expression in cytokine-activated human umbilical vein endothelial cells (HUVECs). Specifically, we have asked a few questions-(i) how long do the cells need to be exposed to the rHDL before adhesion molecule expression is inhibited and (ii) how long does the inhibition persist after removing the rHDL from the cells. When the cells were not pre-incubated with the rHDL, there was no inhibition. The magnitude of the inhibition increased progressively with increasingduration of pre-incubation up to 16 h. Inhibition did not require the rHDLto be physically present during the activation of adhesion molecule expression by tumour necrosis factor(TNF)-alpha, excluding the possibility that the rHDL was merely interfering with the interaction between TNF-alpha and the cells. When HUVECs were pre-incubated for 16 h with rHDL, the inhibitionremained substantial even if the rHDL were removed from the medium up to 8h prior to addition of TNF-a. The HDL-mediated inhibition of VCAM-1 in HUVECs was unaffected by the presence of puromycin, an inhibitor of protein synthesis, excluding the possibility that HDL may have acted by stimulating the synthesis of a cell protein that itself inhibits adhesion molecule expression. These results have important implications in terms of understanding the mechanism(s) of the HDL-mediated inhibition of endothelial adhesion molecule expression. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:18:14