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Titolo:
Comparison between the quality and function of sperm after semen processing with two different methods
Autore:
Hammadeh, ME; Zavos, PM; Rosenbaum, P; Schmidt, W;
Indirizzi:
Univ Saarland, Dept Obstet Gynecol, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 Gynecol, D-66421 Homburg, Germany Androl Inst Amer, Lexington, KY USA Androl Inst Amer Lexington KY USAAndrol Inst Amer, Lexington, KY USA Kentucky Ctr Reprod Med & IVF, Lexington, KY USA Kentucky Ctr Reprod Med &IVF Lexington KY USA & IVF, Lexington, KY USA
Titolo Testata:
ASIAN JOURNAL OF ANDROLOGY
fascicolo: 2, volume: 3, anno: 2001,
pagine: 125 - 130
SICI:
1008-682X(200106)3:2<125:CBTQAF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO FERTILIZATION; INVITRO FERTILIZATION; HUMAN SPERMATOZOA; PERCOLL-GRADIENT; STRICT CRITERIA; PREGNANCY RATES; SWIM-UP; MORPHOLOGY; DNA; FILTRATION;
Keywords:
sperm; semen processing; spermPrep(TM); percoll gradient centrifugation; IVF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Hammadeh, ME Univ Saarland, Dept Obstet Gynecol, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 6421 Homburg, Germany
Citazione:
M.E. Hammadeh et al., "Comparison between the quality and function of sperm after semen processing with two different methods", ASIAN J AND, 3(2), 2001, pp. 125-130

Abstract

Aim: To compare the recovery rate of morphologically normal and chromatin condensed spermatozoa from native semen samples using the SpermPrep (TM) filtration columns and Percoll gradient centrifugation and to determine the influence of the two processing techniques on fertilization and pregnancy rates in an IVF-ET program. Methods: Sixteen semen samples obtained from patient's husband were included in this study. Each was divided into two aliquots. The first aliquot was processed with SpermPrep (TM) filtration columns and the second, Percoll gradient centrifugation. Smears were made before and after semen processing with both methods for the evaluation of chromatin condensation (chromomycine CMA3) as well as morphology (strict criteria) ofspermatozoa. One hundred and seventy oocytes were retrieved from the patients and the oocytes from each patient were subdivided into two sets: one set was inseminated using spermatozoa processed with SpermPrep (TM) and the other inseminated after semen processing with Percoll gradient centrifugation. Results: The Percoll method yielded a significantly higher percentage ofchromatin condensed ((90.8 +/-6.5% vs 82.3 +/-8.8%, P = 0.017) and morphologically normal spermatozoa (12.9 +/-7.1 % vs 6.9 +/-1.8%, P = 0.001) in comparison to SpermPrep (TM). Whereas, sperm count recovery rate was significantly higher after the use of SpermPrep (TM) than after the Percoll gradient centrifugation. The fertilization rate was similar between the two methods. Conclusion: Semen processing with Percoll should be recommended for intracytoplasmic sperm injection as the natural selection is bypassed and the SpermPrep (TM) technique could be recommended for IVF and IUI programs as the sperm concentration plays a more significant role in these procedures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:25:47