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Titolo:
Epidermal growth factor regulation of rat NHE2 gene expression
Autore:
Xu, H; Collins, JF; Bai, LQ; Kiela, PR; Lynch, RM; Ghishan, FK;
Indirizzi:
Univ Arizona, Hlth Sci Ctr, Dept Pediat, Steele Mem Childrens Res Ctr, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 Childrens Res Ctr, Tucson, AZ 85724 USA Univ Arizona, Hlth Sci Ctr, Dept Physiol, Steele Mem Childrens Res Ctr, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 Childrens Res Ctr, Tucson, AZ 85724 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: C504 - C513
SICI:
0363-6143(200108)281:2<C504:EGFROR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+/H+ EXCHANGE ACTIVITY; MOLECULAR-CLONING; EPITHELIAL-CELLS; INTRACELLULAR PH; PARATHYROID-HORMONE; SMALL-INTESTINE; MESSENGER-RNA; H+ EXCHANGE; DIFFERENTIAL REGULATION; FACTOR STIMULATION;
Keywords:
gene regulation; rat intestine; sodium-hydrogen exchanger isoform-2; rat intestinal epithelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Ghishan, FK Univ Arizona, Hlth Sci Ctr, Dept Pediat, Steele Mem Childrens Res Ctr, 1501 N Campbell Ave, Tucson, AZ 85724 USA Univ Arizona 1501 N Campbell Ave Tucson AZ USA 85724 85724 USA
Citazione:
H. Xu et al., "Epidermal growth factor regulation of rat NHE2 gene expression", AM J P-CELL, 281(2), 2001, pp. C504-C513

Abstract

Epidermal growth factor (EGF) is involved in acute regulation of Na+/H+ exchangers (NHEs), but the effect of chronic EGF administration on NHE gene expression is unknown. The present studies showed that EGF treatment increased NHE2-mediated intestinal brush-border membrane vesicle Na+ absorption and NHE2 mRNA abundance by nearly twofold in 19-day-old rats. However, no changes were observed in renal NHE2 mRNA or intestinal and renal NHE3 mRNA abundance. To understand the mechanism of this regulation, we developed the rat intestinal epithelial (RIE) cell as an in vitro model to study the effectof EGF on NHE2 gene expression. EGF increased functional NHE2 activity andmRNA abundance in cultured RIE cells, and this stimulation could be blocked by actinomycin D (a transcriptional inhibitor). Additionally, NHE2 promoter reporter gene assays in transiently transfected RIE cells showed an almost twofold increase in promoter activity after EGF treatment. We conclude that rat NHE2 activity can be stimulated by chronic EGF treatment and that this response is at least partially mediated by gene transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:12:32