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Titolo:
Localization of endogenous and recombinant Na+-driven anion exchanger protein NDAE1 from Drosophila melanogaster
Autore:
Sciortino, CM; Shrode, LD; Fletcher, BR; Harte, PJ; Romero, MF;
Indirizzi:
Case Western Reserve Univ, Dept Physiol & Biophys, Sch Med, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Genet, Sch Med, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Pharmacol, Sch Med, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: C449 - C463
SICI:
0363-6143(200108)281:2<C449:LOEARN>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR PH REGULATION; SODIUM-BICARBONATE COTRANSPORTER; DEPENDENT CL-/HCO3 EXCHANGE; BASE TRANSPORT-SYSTEMS; X-RAY-MICROANALYSIS; MALPIGHIAN TUBULES; V-ATPASE; FUNCTIONAL EXPRESSION; TISSUE DISTRIBUTION; CHLORIDE TRANSPORT;
Keywords:
electrophysiology; fusion proteins; immunohistochemistry; development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Romero, MF Case Western Reserve Univ, Dept Physiol & Biophys, Sch Med, 2119 Abington Rd, Cleveland, OH 44106 USA Case Western Reserve Univ 2119 Abington Rd Cleveland OH USA 44106
Citazione:
C.M. Sciortino et al., "Localization of endogenous and recombinant Na+-driven anion exchanger protein NDAE1 from Drosophila melanogaster", AM J P-CELL, 281(2), 2001, pp. C449-C463

Abstract

Na+-dependent Cl-/HCO3- exchange activity helps maintain intracellular pH (pH(i)) homeostasis in many invertebrate and vertebrate cell types. Our laboratory cloned and characterized a Na+-dependent Cl-/HCO3- exchanger (NDAE1) from Drosophila melanogaster (Romero MF, Henry D, Nelson S, Harte PJ, andSciortino CM. J Biol Chem 275: 24552-24559, 2000). In the present study weused immunohistochemical and Western blot techniques to characterize the developmental expression, subcellular localization, and tissue distribution of NDAE1 protein in D. melanogaster. We have shown that a polyclonal antibody raised against the NH2 terminus of NDAE1 (alpha CWR57) recognizes NDAE1 electrophysiologically characterized in Xenopus oocytes. Moreover, our results begin to delineate the NDAE1 topology, i.e., both the NH2 and COOH termini are intracellular. NDAE1 is expressed throughout Drosophila developmentin the central and peripheral nervous systems, sensilla, and the alimentary tract (Malpighian tubules, gut, and salivary glands). Coimmuno-labeling of larval tissues with NDAE1 antibody and a monoclonal antibody to the Na+-K-ATPase alpha -subunit revealed that the majority of NDAE1 is located at the basolateral membranes of Malpighian tubule cells. These results suggest that NDAE1 may be a key pHi regulatory protein and may contribute to basolateral ion transport in epithelia and nervous system of Drosophila.

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Documento generato il 03/04/20 alle ore 06:54:00